Search found 3 matches

by is5298mih
Thu May 25, 2006 1:17 pm
Forum: Molecular Biology
Topic: RT PCR
Replies: 3
Views: 2053

RT PCR

I am interested in amplifying a 3.0 kb cDNA using primers whose Tm are 67 and 64 C. The template has GC of 55%. the cDNA smear on a gel shows a wide molecular range including the 3.0 kb molecular size fragments. The cDNA positive controls worked as expected. My PCR was done using 0.5U Taq, 200 uM dN...
by is5298mih
Tue Mar 28, 2006 9:18 pm
Forum: Molecular Biology
Topic: Protein Databases
Replies: 1
Views: 1179

Protein Databases

Hello,

I am interested in looking at protein sequences and find out information such as nuclear localization signals, specific domains that may be relevant in transcription and tertiary structure predictions. Any specific online tool(s) you can recommend?

Thank you
by is5298mih
Tue Mar 28, 2006 3:24 pm
Forum: Molecular Biology
Topic: HIS TAG ISSUES
Replies: 2
Views: 1744

HIS TAG ISSUES

Hello, 1. Where would you put a C-terminal HIS tag sequence in a cDNA of interest? (i.e. how close to your stop codon is most favorable). 2. HIS can be transcribed using CAC or CAT. Is it critical to use one versus the other when our aim is to express and purify proteins? 3. If there are two HIS tag...