Search found 34 matches

by SWEngineer
Sat Nov 26, 2011 11:27 am
Forum: Microbiology
Topic: Calculating CFU/g
Replies: 2
Views: 11514

Re:

if 5 grams give 10 cm3, what is the factor to recalculate CFU/cm3 to CFU/g? As I understood after asking, after calculating CFU/cm^3. We now say that this is for (5g/10cm^3), that is for 1/2 dilution, but here, we have to find it as a whole and not as a dilution. So, it will be: result of CFU/cm^3 ...
by SWEngineer
Fri Nov 25, 2011 10:29 am
Forum: Microbiology
Topic: Calculating CFU/g
Replies: 2
Views: 11514

Calculating CFU/g

If we have a 5 gram food sample that was homogenised in sterile Ringer's solution to give a total volume of 10 cm3. After that, if we serially dilute where 0.1 cm3 aliquots of a number of dilutions are spread plated on duplicate nutrient agar plates, and after incubation, we get for example the foll...
by SWEngineer
Thu Nov 24, 2011 12:58 pm
Forum: Microbiology
Topic: Average colony count
Replies: 3
Views: 3234

Average colony count

If I have an agar plate with some dilution, and have made two replicates of that plate. Saw that the number of colonies in replicate (1) was 31 and in replicate (2) 38. Now, in taking the average, we get 34.5

So, in this case, do I say that the average number of colonies is 35 colonies?

Thanks.
by SWEngineer
Sat Nov 19, 2011 3:38 pm
Forum: Cell Biology
Topic: Using the pipette
Replies: 4
Views: 4016

Using the pipette

I just want to confirm the usage of a 50-200 ul autopipette. If I want to obtain a volume of 0.1 ml using such pipette, will the setting used be 050?

Thanks.
by SWEngineer
Fri Nov 18, 2011 9:12 am
Forum: Cell Biology
Topic: Mitochondrial energy pathways
Replies: 9
Views: 10902

I asked my tutor about this, and seems that it is different, and understood that mitochondrial energy pathways are what occur in the mitochondria. That is Krebs cycle and oxidative phosphorylation, excluding glycolisis that occurs in the cytoplasm.
by SWEngineer
Thu Nov 17, 2011 8:55 am
Forum: Cell Biology
Topic: Mitochondrial energy pathways
Replies: 9
Views: 10902

Mitochondrial energy pathways

When we say "mitochondrial energy pathways", is that the same as "cellular respiration pathways"?

Thanks.
by SWEngineer
Thu Nov 17, 2011 8:38 am
Forum: Cell Biology
Topic: Methods of analysis of mtDNA
Replies: 2
Views: 2324

Re:

aptitude wrote:Do you mean genetically, where it is used to determine maternal inheritance?


I'm not sure really, but I think this will be the case, since at the end the results will be compared with a standard.
by SWEngineer
Wed Nov 16, 2011 10:00 pm
Forum: Cell Biology
Topic: Methods of analysis of mtDNA
Replies: 2
Views: 2324

Methods of analysis of mtDNA

What are examples of some of the methods of analysis of mtDNA?

Thanks.
by SWEngineer
Mon Nov 14, 2011 1:54 pm
Forum: Microbiology
Topic: Escherichia coli arrangement
Replies: 1
Views: 2765

Escherichia coli arrangement

What is the arrangement (i.e; irregular groups, in pairs, in chains, ...etc) of Escherichia coli?

Thanks.
by SWEngineer
Mon Nov 14, 2011 12:31 pm
Forum: Microbiology
Topic: Pseudomonas fluorescens morphology
Replies: 1
Views: 2753

Pseudomonas fluorescens morphology

I'm trying to look for information about the Pseudomonas fluorescens morphology (i.e; shape, size, surface, elevation, pigmentation, and edge) but couldn't find clear information on that.

Are there references you recommend where I can find such information?

Thanks.
by SWEngineer
Sat Nov 05, 2011 12:38 pm
Forum: Microbiology
Topic: Classification of Haemolysins
Replies: 1
Views: 3024

Classification of Haemolysins

How are haemolysins classified?

Thanks.
by SWEngineer
Sun Oct 30, 2011 11:40 am
Forum: Microbiology
Topic: Which stain is frequently used to identify Mycobacterium?
Replies: 3
Views: 3510

Re:

JorgeLobo wrote:Ziehl Neelson and its modifications.


Yes, you mean Ziehl–Neelsen stain.

Thanks.