Search found 6 matches

by vlad81
Wed Feb 22, 2012 11:25 am
Forum: Bioinformatics
Topic: fusion gene QPCR primer selection. How?
Replies: 1
Views: 3105

fusion gene QPCR primer selection. How?

Hi All, I am having the following problem. I work with a fusion gene and need to do a QPCR. I found a couple of papers with primers I can use but have no idea how people manage to design those primers and what is the product length etc. therefore I would like to ask you guys if anyone is aware of ho...
by vlad81
Mon Jun 07, 2010 1:18 pm
Forum: Cell Biology
Topic: isoforms in cell line
Replies: 4
Views: 3519

thanks for the response guys, I understand that may question may well be funny, but I am affraid my supervisor is not going to laugh as much when I show him my blots. JackBean, I found the isoforms for the protein on m. For 16 of them it is written " Protein coding", for the rest - "N...
by vlad81
Sun Jun 06, 2010 7:05 pm
Forum: Cell Biology
Topic: isoforms in cell line
Replies: 4
Views: 3519

isoforms in cell line

Hi all, I am working with cell line and looking at a particular protein which has 25 protein coding isoforms from a wide range of molecular weight. Can anyone tell me if there is some max possible isoforms co-expressed in one particular cell line or is it really possible to have all of them, 25, in ...
by vlad81
Sat May 08, 2010 3:06 pm
Forum: Molecular Biology
Topic: Serial Dilution BSA Bradford measurement
Replies: 5
Views: 10184

Re: Serial Dilution BSA Bradford measurement

Well, that was really very helpful. Thanks

Best Wishes JackBean!
by vlad81
Fri May 07, 2010 6:52 pm
Forum: Molecular Biology
Topic: Serial Dilution BSA Bradford measurement
Replies: 5
Views: 10184

Re: Serial Dilution BSA Bradford measurement

Great thank you indeed! Which equation did you use? I was trying to use the C1V1=C2V2 formula 10x stock solution: 15mg/ml (I put 0.0015g in 10ml) then according to the equation: for 1mg/ml I have 15mg/ml x V1 = 1ml x 1mg/ml => V1 = 1/15=0.067ml Thus I take 0.067ml from 10x stock solution and add 0.0...
by vlad81
Thu May 06, 2010 7:17 pm
Forum: Molecular Biology
Topic: Serial Dilution BSA Bradford measurement
Replies: 5
Views: 10184

Serial Dilution BSA Bradford measurement

Dear all, I am experiencing real difficulties with something very simple such as serial dilutions and preparation of appropriate stock solutions. For my Bradford protein measurement I need to prepare 0.1, 0.25, 0.5, 1, 1.5 mg/ml protein. I would also like to prepare stock solution in order to use re...