Search found 5 matches

by testsubjectzz
Fri Mar 30, 2012 11:32 pm
Forum: Cell Biology
Topic: Subcellular Fractionation question
Replies: 1
Views: 2105

Subcellular Fractionation question

You're going to get 4 fractions when you're doing this Cytosolic proteins Membrane proteins Nuclear Proteins Cytoskeletal proteins For example in the Qproteome kit it breaks open the plasma membrane but doesn't solubilise it you spin that down the supernatant are the cytosolic proteins but once you'...
by testsubjectzz
Sat Sep 25, 2010 1:50 pm
Forum: Molecular Biology
Topic: primer designing
Replies: 4
Views: 3792

For designing primers I use SE Central Clone Manager, this tells me hairpins/dimers/runs/melting etc about the primer that I'm designing.
by testsubjectzz
Sat Sep 25, 2010 1:43 pm
Forum: Molecular Biology
Topic: How to determine success of a digest producing short segment
Replies: 4
Views: 2858

I would say it most likely did run off on a 1.5% gel, if it were me trying that I would try a 5% agarose gel also @jackbean for big segments of dna it is best to use a low % gel instead of a high one.
by testsubjectzz
Sat Sep 25, 2010 1:32 pm
Forum: Molecular Biology
Topic: Expression Vector vs Cloning Vector
Replies: 1
Views: 3327

Expression Vector vs Cloning Vector

I have this question how come with cloning why can we not clone directly into the expression vector why is it done in 2 stages?
by testsubjectzz
Thu Apr 29, 2010 11:35 am
Forum: Molecular Biology
Topic: Plasmid mini prep
Replies: 2
Views: 1255

Plasmid mini prep

So I did a plasmid mini prep and you forgot to use RNAse would it be suitable for cloning, i'm guessing not but hoping yes. I will be running a gel tomorrow to check it and i'm pretty sure i'll need to repeat it Right now it's in -20 dissolved in distilled water or something could I possibly centrif...