Search found 67 matches

by HELISA
Thu Jan 22, 2009 4:06 am
Forum: Molecular Biology
Topic: reversion of transitions and transversion
Replies: 0
Views: 1176

reversion of transitions and transversion

Does anyone encounter the general theory explaining the frequency of reversion for transitions and transversion? I’m trying to figure out what could be the probability of reversion for C->T transition that I’ve introduced to one of my constructs. I would appreciate any suggestions or literature refe...
by HELISA
Sat Nov 22, 2008 9:50 pm
Forum: Cell Biology
Topic: Golgi Body
Replies: 1
Views: 4202

Golgi apparatus even though highly dynamic doesn't dissapear because it's recreated over and over again. Cis-cisternes don't dissapear because even though they move during maturation to medial and trans Golgi, what's left behind them is again recreated from ER vesicles. Similarily, trans-Golgi ciste...
by HELISA
Sat Nov 22, 2008 9:44 pm
Forum: Cell Biology
Topic: How does a mutated gene affect Tyrosine Kinase?
Replies: 1
Views: 1876

I believe you are correct, in both instances the signal transduction would be inhibited. 1) the receptor is not able to bind the ligand properly, therefore no conformational change would occur 2) the receptor bound the ligand, but the lack of intracellular domain stopped the further transduction eve...
by HELISA
Sat Nov 22, 2008 9:39 pm
Forum: Cell Biology
Topic: The free energy change question
Replies: 2
Views: 3704

Thank you very much for you help! If anyone has suggestions concerning this problem feel free to write about it!
by HELISA
Thu Nov 20, 2008 11:24 pm
Forum: Cell Biology
Topic: The free energy change question
Replies: 2
Views: 3704

The free energy change question

All right, I'm honest...It's my homework, but I don't ask anyone to do it for me. I did it myself, the only thing is, I'm not sure if it's correct. I would appreciate any suggestions. Here it goes: Calculate the free energy change associated with ATP exiting a cell through one of the ATP permeant tr...
by HELISA
Tue Aug 19, 2008 6:40 pm
Forum: Molecular Biology
Topic: RNA extraction from agarose gel
Replies: 1
Views: 2422

RNA extraction from agarose gel

Does anyone know a good kit to extract RNA from agarose gel ?
The quality of RNA should be good enough to do RT-PCR.
I found one kit online - Zymoclean Gel RNA Recovery Kit - but I'm not sure if it's good.
Does anyone has experience with it?
I appreciate all the suggestions! :)
by HELISA
Tue Jul 08, 2008 5:06 pm
Forum: Molecular Biology
Topic: Are my blots ruined?
Replies: 3
Views: 1504

Re: Are my blots ruined?

Blots are fine, nothing happened ..thank God!
by HELISA
Tue Jul 08, 2008 12:42 pm
Forum: Molecular Biology
Topic: Are my blots ruined?
Replies: 3
Views: 1504

Today, I'm going to check my blots after exposition on X ray film.
Keep your fingers crossed guys!
by HELISA
Thu Jul 03, 2008 4:36 pm
Forum: Molecular Biology
Topic: Are my blots ruined?
Replies: 3
Views: 1504

Are my blots ruined?

I've added formaldehyde instead of formamide to my prehybridization buffer. My blots were incubated for like 1 minute in this solution, and when I realized that something is wrong I promptly removed the buffer, washed the tubes with low stingency wash buffer (SSC and SDS) and replaced the solution ...
by HELISA
Sun Sep 23, 2007 9:22 pm
Forum: Botany Discussion
Topic: Phytoalexins
Replies: 0
Views: 1791

Phytoalexins

Does anyone know publications that say something about the production of phytoalexins by virus-infected plant cells? I've searched the internet, and all the information I got are about the synthesis of phytoalexins by plants infected by bacteria or fungi. I've found one article, saying something abo...
by HELISA
Mon Sep 17, 2007 10:54 pm
Forum: Molecular Biology
Topic: How to differentiate between RNA and DNA
Replies: 12
Views: 12196

LOL

:lol:
that's a good observation!
by HELISA
Thu Sep 13, 2007 2:18 pm
Forum: Molecular Biology
Topic: How to differentiate between RNA and DNA
Replies: 12
Views: 12196

EtBr

I'm sure EtBr intercalate with secondary structures of RNA, so that wouldn't work for my purpose...
How about the use of restriction enzymes? Do you guys know the enzyme which would cut almost every kind of DNA leavind RNA intact?