Search found 34 matches

by simpleton
Thu Oct 13, 2011 2:35 pm
Forum: Cell Biology
Topic: Reduced GFP expression in apoptotic cells?
Replies: 2
Views: 4771

i see. thanks a lot. :)

just not sure if my interpretations are right.
by simpleton
Sun Oct 09, 2011 9:40 am
Forum: Cell Biology
Topic: Reduced GFP expression in apoptotic cells?
Replies: 2
Views: 4771

Reduced GFP expression in apoptotic cells?

Dear all, I carried out an experiment to study apoptosis in cancer cells. In one of the prototype, I observed reduced GFP expression while the GFP expression in control remains the same before incubation for 24hrs. May I know if apoptosis will contribute cause reduction in GFP expression? I supposed...
by simpleton
Tue Nov 23, 2010 3:19 pm
Forum: Molecular Biology
Topic: Extracellular domain or intracellular domain?
Replies: 3
Views: 2625

Hey JackBean! Thanks a lot for your help!

Really appreciate it!

Sadly this is just a theoretical question. I doubt I will be given the opportunity to carry out this experiment :(
by simpleton
Sun Nov 21, 2010 3:24 pm
Forum: Molecular Biology
Topic: Extracellular domain or intracellular domain?
Replies: 3
Views: 2625

Extracellular domain or intracellular domain?

I spent quite a while thinking how to determine which parts of a protein face outward from the plasma membrane and which parts face inwards?

How can I go about determining the intracellular domain and extracellular domain of a new protein?

Can anyone please help? Many thanks in advance!
by simpleton
Fri May 21, 2010 11:17 am
Forum: Genetics
Topic: E coli replication and transcription
Replies: 6
Views: 6122

agree with jackbean that bacteria does not have the programmed life cycle (G1, S, G2 and mitosis phases). as long as the conditions are right, they go ahead to replicate. unlike eukaryotes which requires unchecking of different stages of life cycle. The cell cycle of prokaryotes is simple and fast. ...
by simpleton
Sat May 08, 2010 4:22 pm
Forum: Genetics
Topic: polycistronic = multigenic
Replies: 5
Views: 6355

Multigenic Description: Many genes are involved in the expression of a trait. Source: Specialized encyclopedia and dictionaries Polycistronic messages have multiple ORF's in one transcript and also have small regions of untranslated sequence between each ORF. But this multigenic definition (as also ...
by simpleton
Sat May 08, 2010 4:08 pm
Forum: Genetics
Topic: polycistronic = multigenic
Replies: 5
Views: 6355

it's really confusing! Orome, i did google but i found different definitions and can't conclude. and in the book, principles of genetic it says that multigenic means that the mRNA contains the coding regions of two or more genes. Then in a lecture note from a Uni, the lecture note wrote polycistroni...
by simpleton
Thu May 06, 2010 7:59 am
Forum: Genetics
Topic: polycistronic = multigenic
Replies: 5
Views: 6355

polycistronic = multigenic

Does polycistronic means multigenic? What I understood was polycistronic means that a mRNA molecule code for multiple proteins with spacer and overlapping genes while multigenic just means that a mRNA codes for multiple proteins (no overlapping between genes)? but then i see that many reference use ...
by simpleton
Thu May 06, 2010 7:55 am
Forum: Genetics
Topic: lac operon
Replies: 4
Views: 3027

conclusion is.. our prof can be quite ambiguous with his questions! :P
by simpleton
Sat May 01, 2010 12:17 pm
Forum: Genetics
Topic: Genetics
Replies: 11
Views: 4879

cause according to "Principles of genetics", it says it is the cross between individuals of same ancestry. :D
by simpleton
Sat May 01, 2010 4:27 am
Forum: Genetics
Topic: Genetics
Replies: 11
Views: 4879

hey jwalin,i chanced upon the correct term for F1/F1. it's called intercross. :)
by simpleton
Fri Apr 30, 2010 8:54 am
Forum: Genetics
Topic: How many gametes?
Replies: 5
Views: 2367

haha of course. :)