Search found 18 matches

by doctobe
Fri Mar 05, 2010 6:12 pm
Forum: Molecular Biology
Topic: Difference between? Complementaton and Recombination
Replies: 7
Views: 8304

Re:

that is something different. I'm talking about histidine kinases, which work as receptors and thus, if you have knock-out of one of them, you can supplement it with plant histidine kinase I went to my teacher yesterday and it seems I was remembering examples versus concepts. I though Ecoli was used...
by doctobe
Fri Mar 05, 2010 6:10 pm
Forum: Molecular Biology
Topic: Differences:Cloning vs sub-cloning
Replies: 3
Views: 6578

Re:

http://en.wiktionary.org/wiki/subclone views doesn't mean much, we have robots looking through all our posts(not kidding). Thank you. I think this helps more. I didn't realize wiki also had a dictionary. Wikipedia's definition for subcloning was my definition for cloning. I was also having trouble ...
by doctobe
Thu Mar 04, 2010 4:37 pm
Forum: Molecular Biology
Topic: Differences:Cloning vs sub-cloning
Replies: 3
Views: 6578

Good amount of views and no replies? I even gave my definition. Open to correction , anything.
by doctobe
Thu Mar 04, 2010 4:36 pm
Forum: Molecular Biology
Topic: Difference between? Complementaton and Recombination
Replies: 7
Views: 8304

Re:

JackBean wrote:they are quite similar, so they can supplement each other. This system is commonly used in such situations.
There is system in both E.coli and Saccharomyces


it is the Ecoli system that was given as an example in class. Trying to understand that one. with the B strain K strain.
by doctobe
Thu Mar 04, 2010 3:33 am
Forum: Molecular Biology
Topic: Differences:Cloning vs sub-cloning
Replies: 3
Views: 6578

Differences:Cloning vs sub-cloning

Okay do DNA cloning is ability to construct recombinant DNA molecules and maintain them in cells. Right. A DNA insert that was cut with restriction enzymes is inserted into a plasmid vector also digested with the same restriction enzymes. Then the plasmid vector is transformed into bacterial cells. ...
by doctobe
Thu Mar 04, 2010 3:11 am
Forum: Molecular Biology
Topic: Define: wild type, Recombinant, Mutant
Replies: 4
Views: 4694

I think as I am doing homework and or reading some of these complementation tests and homework problems being given. It is difficult to understand the difference between a mutant and recombinant. Also difficult when the recombinant produced it used as a parent along with wild type to attempt to prov...
by doctobe
Thu Mar 04, 2010 2:59 am
Forum: Molecular Biology
Topic: Difference between? Complementaton and Recombination
Replies: 7
Views: 8304

Re:

I think, that complementation and recombination has nothing to do basically. Recombination is exchange of genetic material between two DNA molecules (chromosomes, plasmids etc.), whereas complementation is process, when you add some gene to compensate for missing endogennous gene. E.g. bacteria nee...
by doctobe
Tue Mar 02, 2010 3:47 am
Forum: Molecular Biology
Topic: Difference between? Complementaton and Recombination
Replies: 7
Views: 8304

Difference between? Complementaton and Recombination

In light of the Complementation test performed by Benzer on T4 phages, what is the difference between complementation and recombination?
(Can you have complementation and not have recombination, can you have complementation and have recombination?
by doctobe
Tue Mar 02, 2010 2:54 am
Forum: Molecular Biology
Topic: Define: wild type, Recombinant, Mutant
Replies: 4
Views: 4694

Define: wild type, Recombinant, Mutant

Hello,

It is sad but I am having trouble trying to define the three terms


1. A recombinant

2.Mutant

3. Wild Type.

There names don't help. Because wild type means "normal", but what the heck is normal in molecular biology?
by doctobe
Sat Feb 20, 2010 8:48 pm
Forum: Molecular Biology
Topic: DNA structure questions (simple)
Replies: 2
Views: 20600

Re:

I think, that the length should be 603 bp, because you have also STOP codon 3) yes, the protein would be able to bind only with one helix. Imagine, that you insert only one nt, only one! What does it cause? Shift by 0.34 nm, that's not so bad, but you have also the twist by 36° and that's more seri...
by doctobe
Sat Feb 20, 2010 6:52 pm
Forum: Molecular Biology
Topic: DNA structure questions (simple)
Replies: 2
Views: 20600

DNA structure questions (simple)

I had some DNA questions. kind of straightfoward I posted what I believe to be the right answers. I wanted to make sure I was reading the questions properly. The twist of a DNA double helix corresponds to the angular rotation that is needed to get from one base pair to the next. In an idealized DNA ...
by doctobe
Sat Feb 20, 2010 6:31 am
Forum: Molecular Biology
Topic: DNA sequenced. Erased labels. Now re-identify.
Replies: 12
Views: 5719

Re:

You can use more, of course (although more than two are not used much probably), but you have to check where will it cut around. But you should not use EcoRI and Tsp at once! Because than you would got two bands in both cases. Okay So I could just cut one sequence with ECO R1 and then do a southern...