Search found 9 matches

by JTeighty
Thu May 06, 2010 8:45 pm
Forum: General Discussion
Topic: % light transmission to enzyme activity graph
Replies: 4
Views: 2467

Re: % light transmission to enzyme activity graph

Ah I see. Thanks a lot :) :)
by JTeighty
Thu May 06, 2010 1:27 pm
Forum: General Discussion
Topic: % light transmission to enzyme activity graph
Replies: 4
Views: 2467

Re:

what are you doing? What is your graph? I think, that you want to convert transmission to absorbance... I'm measuring effect of acid concentrations on an enzyme. I used percentage transmission as gave better results. On x axis is concentration an y axis is % light transmission. I want to make y axi...
by JTeighty
Wed May 05, 2010 3:54 pm
Forum: General Discussion
Topic: % light transmission to enzyme activity graph
Replies: 4
Views: 2467

% light transmission to enzyme activity graph

Hi,

I have drawn a graph of percentage light transmission for an enzyme (postivite correlation) and I want to change this into a graph showing enzyme activity (should be a negative correlation). How can I do this? Would inversing the graph work?

Thanks

JT80
by JTeighty
Tue Apr 20, 2010 9:37 pm
Forum: Molecular Biology
Topic: Catechol Oxidase, Polyphenol Oxidase and Tyrosinase
Replies: 6
Views: 12301

Catechol Oxidase, Polyphenol Oxidase and Tyrosinase

Hi,

Are Catechol Oxidase, Polyphenol Oxidase and Tyrosinase the same enzyme?

Many thanks

JT80
by JTeighty
Thu Mar 04, 2010 8:06 am
Forum: Molecular Biology
Topic: Benzoic acid and Ascorbic acid on enzymes
Replies: 4
Views: 3577

Re:

JackBean wrote:
(but I think, that denaturation is not taken as inhibition)


Thanks. That's a good point.
by JTeighty
Wed Mar 03, 2010 8:05 am
Forum: Molecular Biology
Topic: Benzoic acid and Ascorbic acid on enzymes
Replies: 4
Views: 3577

Re: Benzoic acid and Ascorbic acid on enzymes

Hi,

thanks for the response. I used that website to find out that they do inhibit the enzyme but my question was how do they usually inhibit enzymes? Do they have a sort of mechanism? Eg do they denature the enzymes by deforming the active site?
by JTeighty
Tue Mar 02, 2010 9:12 pm
Forum: Molecular Biology
Topic: Benzoic acid and Ascorbic acid on enzymes
Replies: 4
Views: 3577

Benzoic acid and Ascorbic acid on enzymes

Hi,

How does benzoic acid inhibit bacterial growth and act as a preservative? Will this have anything to do with the way it acts as an enzyme inhibitor? How does it actually inhibit the enzyme? What is the mechanism? Is it the same for Ascorbic acid?

Thanks

JT80
by JTeighty
Wed Feb 10, 2010 6:38 pm
Forum: Molecular Biology
Topic: Enzyme inhibition
Replies: 2
Views: 1970

Re:

JackBean wrote:pH and temperature are not much specific and they are not technically inhibitors

If you want to find inhibitors of your enzyme, look here:
http://www.brenda-enzymes.org/


Thanks a lot :-)
by JTeighty
Wed Feb 10, 2010 1:55 pm
Forum: Molecular Biology
Topic: Enzyme inhibition
Replies: 2
Views: 1970

Enzyme inhibition

Hi, Do enzymes get denatured/inhibited by common preservatives such as sorbic acid or does sorbic acid only work against microorganisms? Take Catechol Oxidase an an example. Would sorbic acid inhibit the enzyme? What else would inhibit them besides the obvious ones e.g. pH, temperature... Thanks JT80