Search found 11 matches

by RuNkLi
Thu Nov 13, 2008 9:07 am
Forum: Microbiology
Topic: Inhibition of RNA/DNA/protein synthesis
Replies: 1
Views: 2139

Inhibition of RNA/DNA/protein synthesis

hi, I want to ask if there is a way to detect inhibition of macromolecules in bacteria by antibiotics with an non radioactive method. normally we use tritium-labelled leucine, thymidine, uridine. I know that these radioactive precursors are very sensitive, but is there a non radioactive method? Can ...
by RuNkLi
Wed Jun 18, 2008 9:40 am
Forum: Microbiology
Topic: Binding of peptide to bacteria
Replies: 1
Views: 2044

Binding of peptide to bacteria

I have wt and mutant bacteria lacking membrane ancored lipid.

The peptide binds to a certain lipid on the bacterial surface.

How can i smartly show, that this peptide binds less to the mutant when compared with the wildtype?

Which method is here the optimal resolution?
by RuNkLi
Mon Jun 16, 2008 10:04 am
Forum: Microbiology
Topic: Bacteria on skin, LOG or STAT?
Replies: 1
Views: 2873

Bacteria on skin, LOG or STAT?

Lately we discussed in our group, if bacteria like s. aureus on skin are present as stationary bacteria or growing bacteria e.g. in logarithmic phase?

surely you can't show this, but for me it is logically that they are present on skin as stationary bacteria.

what do you guys think about it?

THX
by RuNkLi
Thu Apr 03, 2008 11:29 am
Forum: Microbiology
Topic: Annexin V staining of bacteria
Replies: 0
Views: 1721

Annexin V staining of bacteria

Hi,

has anyone tried to make FACS analysis with Annexin V and bacteria, to look for apoptosis in bacteria (after treatment with antimicrobial peptide) ?

Even if it is crazy, i want to try :)
by RuNkLi
Thu Apr 03, 2008 11:26 am
Forum: Bioinformatics
Topic: Structure prediction???
Replies: 1
Views: 2606

Structure prediction???

Hi, i have just found a peptide fragment of a LamaG protein which is 11 aminoacids long. I want to ask, if it is possible to make tertiary structure prediction and alpha helical wheel blott with 11 aminoacids: GRWHKVSVRWE Can anyone help me with the data? I want to publisch some facts of this peptid...
by RuNkLi
Mon Jun 04, 2007 9:32 pm
Forum: Microbiology
Topic: Fractination of SA into Cytosol., Cell Wall, Cytoplasmic
Replies: 3
Views: 6859

First thank you very much for your detailed answer. In immune electronmicroscopy pictures we can see, that this peptide binds to surfacial proteins as well as cytoplasmic structures?. We want to confirm, make sure and find the mode of action by finding out these targets. The point you mention is ver...
by RuNkLi
Mon Jun 04, 2007 2:39 pm
Forum: Microbiology
Topic: Fractination of SA into Cytosol., Cell Wall, Cytoplasmic
Replies: 3
Views: 6859

Fractination of SA into Cytosol., Cell Wall, Cytoplasmic

Hi, i have to seperate protein fractions of cytosolic proteins, cell wall proteins and cytoplasmic proteins. the reason is, i want to find out where the antimicrobial peptide is located after incubation with S. aureus. I just control with Western Blot. The problem is, i can't find a protocol for iso...
by RuNkLi
Mon Jun 04, 2007 2:32 pm
Forum: Microbiology
Topic: antimicrobials
Replies: 2
Views: 2360

antimicrobial peptides for example which are positivly charged can interact with the negativley charged bacterial membrane (forming pores). so this can be the first step of mode of action of the antimicrobial peptide.

selamlar

ilknur
by RuNkLi
Thu Feb 09, 2006 11:10 am
Forum: General Discussion
Topic: Can YOU or anyone help me?
Replies: 4
Views: 8385

Can YOU or anyone help me?

come on, i posed this thread some time ago, but no one answerd me my question: "so i have the question if in case of macromolecular synthesis. the cultivation of a bacterium lets say staph aureus in this experiment is denoted in many protocols in phosphate buffer...but when they do not grow in ...
by RuNkLi
Tue Jan 31, 2006 11:08 am
Forum: Microbiology
Topic: Macromolecular Synthesis
Replies: 5
Views: 4915

In English is may better so i have the question if in case of macromolecular synthesis. the cultivation of a bacterium lets say staph aureus in this experiment is denoted in many protocols in phosphate buffer...but when they do not grow in the lag phase how can tritium labeled -thymidine or uridine ...
by RuNkLi
Tue Jan 31, 2006 11:01 am
Forum: Microbiology
Topic: Macromolecular Synthesis
Replies: 5
Views: 4915

Macromolecular Synthesis

Ich bin gerade dabei ein Protokoll zu erstellen für ein Mikroorganismus, dass nach Zugabe von Antibiotika auf DNA/RNA/Protein-Ebene mittels Tritium-markierten Metaboliten untersucht werden soll... Meine Frage wäre, müssen sich die MO während des Einbaus von Metaboliten wie Thymidin,Uridin,Leucin in ...