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by gael
Tue Nov 03, 2009 10:33 am
Forum: Cell Biology
Topic: antibody staining
Replies: 2
Views: 2641

antibody staining

Hi, I'm doing immunofluorescence in vitro and in vivo, and i found a new subcellular location for a protein with one antibody (which has been use in many publication). Pre-incubation with the immunogen peptide (blocking peptide) result in the loss of the signal. Overexpression of this protein in cel...