Search found 4 matches

by cmgross
Thu Nov 05, 2009 9:14 pm
Forum: Molecular Biology
Topic: CsCl density centrifugation
Replies: 1
Views: 1131

CsCl density centrifugation

Does CsCl centrifugation allow you to separate circular DNA from linear DNA?
by cmgross
Wed Nov 04, 2009 6:19 am
Forum: Molecular Biology
Topic: replication intermediates
Replies: 1
Views: 1138

replication intermediates

I have a ds linear viral DNA genome of 100 kb. It has been determined that the replication intermediate is a 200 kb dsDNA circle. How can you expain the structure/transition to the replication intermediate of a 200 kb circle?
by cmgross
Wed Nov 04, 2009 6:13 am
Forum: Zoology Discussion
Topic: ciliates and telomerase
Replies: 0
Views: 1386

ciliates and telomerase

Is there a reason why ciliates are a good place to study telomerase/telomeres?
by cmgross
Sat Oct 31, 2009 3:49 pm
Forum: Molecular Biology
Topic: distinguishing circular and linear DNA
Replies: 9
Views: 17733

distinguishing circular and linear DNA

I need to learn at least 2 different ways to determine if a 100 kb ds DNA viral genome is circular or linear. We are starting with highly purified viral DNA in a tube. I know that circular and linear DNA run differently on a gel, but I don't think you can run fragments that big on an agarose gel. Th...