Search found 21 matches

by MJD
Fri Sep 17, 2010 2:13 pm
Forum: Molecular Biology
Topic: How to determine success of a digest producing short segment
Replies: 4
Views: 3499

Re: How to determine success of a digest producing short segment

You likely ran off your 45bp segment of the 1.5% agarose gel. You might consider running a more concentrated gel like a 3.5 agarose gel to resolve the 45bp segment and the apparent difference between the linearized vector and the doubly digested linearized vector. I suggest you also run the undigest...
by MJD
Fri Aug 20, 2010 10:53 pm
Forum: General Discussion
Topic: List of peer reviewed journals
Replies: 6
Views: 14349

Re: List of peer reviewed journals

Below is a link to open access (freely accessible to the public) peer reviewed journals. You can find Biology and Medicine Journals under relevant titles. http://ucblibraries.colorado.edu/dean/peer_reviewed.htm
by MJD
Thu Nov 05, 2009 9:12 am
Forum: Cell Biology
Topic: FBS store
Replies: 11
Views: 17765

Re: FBS store

First of all, like other have said, I think the serum should be fine left out overnight. However, I'll state that I have cultured cells for a long time without having "inactivated" the FBS serum. Additionally, I believe the term inactivation is a misnomer and the proposed function is to re...
by MJD
Wed Oct 07, 2009 2:22 am
Forum: Molecular Biology
Topic: Western transfer failure
Replies: 5
Views: 10397

Re: Western transfer failure

Well the simplest fix is that you are putting the cassette or gel to nitrocellulose in backwards. When you do this, the protein is pushed away from the nitrocellulose and there will be absolutely nothing including the ladder on the nitrocellulose membrane. Two ways to remember this is black to black...
by MJD
Sat Oct 03, 2009 8:57 pm
Forum: General Discussion
Topic: Can any one help me explain this?
Replies: 2
Views: 13557

Re: Can any one help me explain this?

Sure. See the link below. It is a powerpoint explaining the questions your have.

https://webspace.utexas.edu/mjd57/www/C ... cation.ppt
by MJD
Thu Oct 01, 2009 2:58 am
Forum: Bioinformatics
Topic: SDS-PAGE help please
Replies: 6
Views: 17880

Re: SDS-PAGE help please

Okay. So it seems that there is a little confusion about what we are taking about. Electrophoresis is a process used for the separation of cellular proteins (polyacrydimide) and DNA (agarose gels). SDS-PAGE is electrophoresis for proteins that utilizes SDS and denaturing conditions to linearize the ...
by MJD
Thu Oct 01, 2009 1:38 am
Forum: Physiology
Topic: Stomach bloating and weight gain
Replies: 457
Views: 3347817

Re: Stomach bloating and weight gain

Wow guys that's terrible. Gastrointestinal problems can really turn your world upside down, especially when no one can give you a valid explanation. Some people have many intolerances and/or allergies to foods. I had gluten intolerance, otherwise known, as Celiac Disease, for 3 years before I self-d...
by MJD
Thu Oct 01, 2009 1:14 am
Forum: Zoology Discussion
Topic: Insect ID please.
Replies: 3
Views: 3926

Re: Insect ID please.

Ichneumonidae; yeah you got it. They have a characteristic horse head in the wing structure, which makes them easy to identify.

See this one http://bugguide.net/node/view/234419
by MJD
Thu Oct 01, 2009 1:09 am
Forum: Zoology Discussion
Topic: Why are the offsprings of some animals large but some small?
Replies: 9
Views: 6817

Re: Why are the offsprings of some animals large but some small?

Resource allocation. A balance of the costs of producing an offspring, caring for an offspring, and the chance that it will survive in the environment. Cost to Benefit Ratio. For instance, some animals produce many offspring at once, which increases the probability that at least or two will survive....
by MJD
Thu Oct 01, 2009 1:03 am
Forum: Zoology Discussion
Topic: Caterpillar I.D.
Replies: 1
Views: 1782

Re: Caterpillar I.D.

Wow! Nice Picture. That's an awesome caterpillar. Consider submitting it to bugguide.net and whatsthatbug.com for identification. Cheers.
by MJD
Thu Oct 01, 2009 12:50 am
Forum: Zoology Discussion
Topic: What is this bug?
Replies: 4
Views: 6470

Re: What is this bug?

Cockroach nymph indeed!

Order: Blattodea
Suborder:Blattaria
Family: Blattellidae
Genus: Blattella


http://bugguide.net/node/view/316311

Check out bugguide.net for all your bug identifying needs.
by MJD
Thu Oct 01, 2009 12:29 am
Forum: Zoology Discussion
Topic: What is this spider?
Replies: 1
Views: 2517

Re: What is this spider?

Go to Bugguide.net

You can look for the identity of the spider there or you can submit the image to bugguide.net and someone will be able to identify if for you in time.

Cheers.