Search found 655 matches

by jwalin
Fri Aug 30, 2013 2:26 pm
Forum: Cell Biology
Topic: Passage number
Replies: 1
Views: 2067

Hi, There are different opinions about this but primarily it depends on the cells that you are growing. In differentiated cell lines the cells wont get reclocked at all. In the lab I work in, we dont consider that the cells can be reclocked. The best way of labelling would be labeling the cell lines...
by jwalin
Fri Aug 30, 2013 2:22 pm
Forum: Cell Biology
Topic: Culturing or viewing cells
Replies: 3
Views: 9712

2. Different cancer cells will definitely require different media. But the general procedure assuming you need to get cells from a particular tissue would be to get the tissue, extract the cells, digest ECM..., try culturing them in a flask / Tissue culture plate (depending on the type of cells it c...
by jwalin
Fri Aug 30, 2013 2:10 pm
Forum: Off Topic Discussion
Topic: Last person to reply wins.
Replies: 8431
Views: 2028884

Its been so long since I have won this game, didnt get anything specific by winning but then if its the moment that counts, then I win.
I know this win wont last too long. :P

Note to self - once a winner always a winner! ;)
by jwalin
Wed Aug 28, 2013 10:21 am
Forum: Molecular Biology
Topic: molecular question.
Replies: 5
Views: 3842

knowing the organism is crucial but given that its a general question a general answer would be that you would use a nucleotide blast (given that you have the gene sequence).
by jwalin
Mon Nov 28, 2011 7:19 pm
Forum: Molecular Biology
Topic: Splicing isoforms
Replies: 4
Views: 4632

Seemed so to me too. Thank you so much :)
by jwalin
Wed Nov 23, 2011 7:28 pm
Forum: Molecular Biology
Topic: Splicing isoforms
Replies: 4
Views: 4632

I am not very sure now it seems so. But yes a clarification would help.
Thanks though. :)
by jwalin
Wed Nov 23, 2011 1:43 pm
Forum: Molecular Biology
Topic: Splicing isoforms
Replies: 4
Views: 4632

Splicing isoforms

I do not understand what is meant by splice isoforms. I used to think that they are the products of alternate splicing. But I was reading a paper on: "mTORbeta Splicing Isoform Promotes Cell Proliferation and Tumorigenesis" And I got confused and decided to get the basic definition right. ...
by jwalin
Wed Apr 20, 2011 1:13 am
Forum: Off Topic Discussion
Topic: Last person to reply wins.
Replies: 8431
Views: 2028884

Ahh back I am. :)
by jwalin
Tue Apr 19, 2011 4:03 am
Forum: Cell Biology
Topic: Cell Division Questions
Replies: 3
Views: 12059

For Q2 D is right for in meiosis 1 there is a separation of homologous chromosomes and not chromatids.
by jwalin
Mon Apr 18, 2011 12:18 pm
Forum: Off Topic Discussion
Topic: Last person to reply wins.
Replies: 8431
Views: 2028884

I wiiiinnnn
by jwalin
Sun Apr 17, 2011 10:39 am
Forum: Cell Biology
Topic: Campbell and Reece 8th edition
Replies: 36
Views: 38994

If you must have noticed the proteins travel from one organelle to another. This transport is made possible by the vesicles. Now one can ask why does this have to be so? Its simple a protein needs to reach a specific place for its post translational modification and there must be something that must...
by jwalin
Mon Jan 03, 2011 6:25 pm
Forum: Off Topic Discussion
Topic: Last person to reply wins.
Replies: 8431
Views: 2028884

I am the next one i presume lol