Search found 10 matches

by scholesmu
Wed Nov 14, 2012 5:11 pm
Forum: Molecular Biology
Topic: Measure of Cysteine concentration
Replies: 2
Views: 3350

Measure of Cysteine concentration

i have no idea how the route of the calculation. 1. 2.6 ml UV-assay buffer add to cuvette 2. 50 ul sample add to curvette and measure absorbance 324 nm (A324 nm of sample) 3. 200 ul DTDP solution add to curvette. If the absorbance is greater than 1, repeat 2 and 3 with less starting sample. Measure ...
by scholesmu
Thu Aug 18, 2011 3:05 pm
Forum: Molecular Biology
Topic: Isotonic solution with physiological saline...
Replies: 2
Views: 3926

Isotonic solution with physiological saline...

an isotonic solution contains 300 mmol/L


if i need to know the molar concentration of

for example NaCl, glucose

to prepare Isotonic solution with physiological saline


is that like to have to count the total number of molecules first?
what to do?
by scholesmu
Thu Apr 28, 2011 12:44 am
Forum: Molecular Biology
Topic: Basic electrophoresis digest banding pattern analysis....
Replies: 5
Views: 6264

Basic electrophoresis digest banding pattern analysis....

I want to know more about banding pattern analysis...... lane1: standard makers (HindIII digest, sizes are 23.1,9.4,6.6,2.3,0.6,0.1kb) lane2: undigested plasmid X lane3: plasmid X with restriction enzyme SciI by student A lane4: plasmid X with restriction enzyme SciI by student B lane5: plasmid X wi...
by scholesmu
Mon Apr 25, 2011 10:23 pm
Forum: Molecular Biology
Topic: DNA restriction map drawing...
Replies: 1
Views: 2670

DNA restriction map drawing...

1. A 1 in 4 dilution of an overnight culture of E coli has an absorbance (590nm) of 0.25 How much overnight culture must be added to 50ml of fresh medium to give a starting culture with absorbance (590nm) of 0.05? 2. A small DNA molecule was cleaved with a number of different restriction endonucleas...
by scholesmu
Sat Jun 19, 2010 4:31 pm
Forum: General Discussion
Topic: calculate the mole of substrate in assay
Replies: 3
Views: 3578

Re: calculate the mole of substrate in assay

thanks...
do you know how to calculate? i am stuggling...
by scholesmu
Fri Jun 18, 2010 3:19 pm
Forum: General Discussion
Topic: calculate the mole of substrate in assay
Replies: 3
Views: 3578

calculate the mole of substrate in assay

"alkaline phosphate is assayed at concentration 1ug/ml at r.t. in 0.1M glycine buffer, pH9.5 in presence of 5mM MgCl2 as a factor in reaction. The substrate 4-nitrophenol (4-NPP) is added in concentration range 0.05 mM to 3.0mM. The appearance of the product, 4-nitrophenol (4-NP) can be moitore...
by scholesmu
Sun Aug 16, 2009 11:49 am
Forum: Molecular Biology
Topic: [molecular bio]calculation
Replies: 3
Views: 7370

Re: [molecular bio]calculation

thanks, please i have one more... stock solutions and powders available: NaCl powder (molecular weight 58.4), 1 M potassium phosphate, and a 5 mg/ml stock of cholesterol (mol. wt. 386.7) Acetic acid (CH3COOH) is a liquid at room temperature. When making acetate buffers (as in the question above) you...
by scholesmu
Sat Aug 15, 2009 9:48 am
Forum: Molecular Biology
Topic: [molecular bio]calculation
Replies: 3
Views: 7370

[molecular bio]calculation

1. A reaction has been design to be carried out in 4ml total volume. It requires a final phenylalanine concentration of 5mM. If the protocol allows 50 ul for the phenylalanine addition, what concentration would you make up the stock solution? 2.A particular cell line will grow well in a standard med...
by scholesmu
Fri Jun 12, 2009 5:21 am
Forum: General Discussion
Topic: lots of questions of various topics........
Replies: 3
Views: 5379

Re: lots of questions of various topics........

yes for question 1, i have no idea staining make the cell more distinct,isn't it?but what is the actual thoery behind this? question 2 i think the answer is either a or b question 4, i only know that second metablites are something that inhibit the growth of surrounding organisms....so is it/ what i...
by scholesmu
Thu Jun 11, 2009 3:25 am
Forum: General Discussion
Topic: lots of questions of various topics........
Replies: 3
Views: 5379

lots of questions of various topics........

i am unsure about these questions, please help, thanks 1. under light microscope, structures are seen more clearly in stained cell than unstained cell,becasue a)many cytoplasmic structures are colourless and can't be distinguished b)staining improves the resolving power of microscope c)cells are alw...