Search found 3278 matches

by canalon
Sat Nov 02, 2013 6:15 pm
Forum: Molecular Biology
Topic: How
Replies: 4
Views: 4257

We would need a bit more details of what you want to do. But technically if you just want to have 2genes one after the next to insert you could either plasmids with multiple insertion sites, make your construction in those (easy) then excise the double insert and transfer it to the genomic DNA as yo...
by canalon
Tue May 28, 2013 2:15 am
Forum: Human Biology
Topic: Brain size=IQ level theory (Blacks vs Whites & Asians)
Replies: 81
Views: 469189

Re: Re:

^ You may wanna check your quotes, I don't think it was Canalon who wrote what you quoted above. Yeah, after I caught that it was too late to change it. Maybe Canalon can fix it. I'd hate to give the impression that it's his quote. I came back from my long disappearance and fixed your problems. But...
by canalon
Thu Jul 26, 2012 2:57 am
Forum: Molecular Biology
Topic: extra unknown band after restriction digestion?
Replies: 11
Views: 8489

NOt sure what we are looking at in each lane. But my guess, since it appears that you have loaded a ton of DNA in your gel (considering the look of the band), would be trace amount of genomic DNA.
by canalon
Thu Jul 26, 2012 2:54 am
Forum: Cell Biology
Topic: bacterial culture growth
Replies: 1
Views: 2674

Never, ever did that. Sounds strange to me. Considering that they will be washed multiple times in plenty of different solutions and buffer any way...
by canalon
Mon Jul 23, 2012 2:10 am
Forum: Human Biology
Topic: Poisoned by antibiotics...can anyone help?
Replies: 23
Views: 117041

In surgery you can receive more than just antibiotics. And a 50 lbs error is quite large (20 kg is a huge difference). I am quite surprise though that she was not weighed before the surgery. But hopefully the excess of those drugs will be metabolized. But the docs are probably the one to ask for det...
by canalon
Mon Jul 16, 2012 4:31 pm
Forum: Molecular Biology
Topic: How to pronounce ""SYBR"
Replies: 2
Views: 2025

I usually hear said As "Cyber"
by canalon
Mon Jul 16, 2012 2:27 am
Forum: Molecular Biology
Topic: restriction analysis
Replies: 14
Views: 12967

by canalon
Sat Jul 14, 2012 4:07 am
Forum: Bioinformatics
Topic: searching for gene sequences, automated
Replies: 8
Views: 6696

Geneious is great if you have a few thousand dollars that do not know how to use :(
I do not know of any free program doing that, but that is more likely a product of my ignorance.

As for what book in the bookshelf, I do not which one for sure, but it is likely one of those: http://www.ncbi.nlm.nih.gov/books?term=pubmed
by canalon
Thu Jul 12, 2012 1:22 am
Forum: Bioinformatics
Topic: searching for gene sequences, automated
Replies: 8
Views: 6696

Yes a lot.
If you look in the NCBI bookshelf they will explain you how to program that for yourself, and many if not most DNA analysis package do provide that option.
by canalon
Thu Jul 05, 2012 3:18 am
Forum: Evolution
Topic: Evolving non "life-or-death" but useful characteristics
Replies: 5
Views: 9026

First, as pointed earlier, life and death is not the only selection. It is more different reproductive success (directly linked to survival, but not only). And in addition you have to think about all the non selective aspect of life (all the random events that can alter a population without much sur...
by canalon
Wed Jul 04, 2012 3:21 am
Forum: Molecular Biology
Topic: DNA extraction from Human blood
Replies: 3
Views: 3555

First you need an orange filter between your camera and your gel. It will tremendously improve your image.

Second, in absence of any reference (Molecular weight marker or anything else, or even explanation of how the gel was run it is hard to say how bad (or not) your result is.