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by kirupha
Wed Mar 07, 2018 8:54 pm
Forum: Molecular Biology
Topic: BAC DNA purification
Replies: 0
Views: 3687

BAC DNA purification

Hi all, I am trying to purify BAC DNA (106 kb) using Qiagen large construct kit. I am able to purify 5-10 ug of DNA but when I run on 0.8 % agarose gel I see a band near the well. After restriction digestion also I see this band but I can detect it with southern. But we need a clean prep to give it ...