Search found 3 matches

by macpat
Sat Jan 13, 2018 5:49 pm
Forum: Molecular Biology
Topic: Does Contamination by Agar Affect PCR?
Replies: 0
Views: 3201

Does Contamination by Agar Affect PCR?

I'm growng cyanobacteria on agar plates using BG11sub0 media. Does anyone know of a specific protocol for DNA extraction when using agar? Will contamination by agar inhibit or affect the PCR or DNA extraction at all? References?
by macpat
Tue Jan 02, 2018 9:52 pm
Forum: Microbiology
Topic: Separation of cyanobacteria based on morphology?
Replies: 1
Views: 2359

Re: Separation of cyanobacteria based on morphology?

I should have included that I plan to detach the cyanobacteria from the moss leaves into sterile water by vortexing in a plastic tube.
by macpat
Tue Jan 02, 2018 9:06 pm
Forum: Microbiology
Topic: Separation of cyanobacteria based on morphology?
Replies: 1
Views: 2359

Separation of cyanobacteria based on morphology?

I'm currently working on a research project which aims to identify cyanbacterial species present on moss leaves through morphological and molecular methods (polyphasic approach to cyanobacterial identification). The genera in question are Nostoc, Stigonema, Cylindrospermum, and Calothrix. My questio...