Search found 23 matches

by Vmedvil
Wed Sep 27, 2017 8:03 am
Forum: Microbiology
Topic: Why doesn't cow manure promote the growth of water mould?
Replies: 1
Views: 1533

The only thing I can think about is that when you add, cow manure that it is too acidic to neutral for them to grow with a PH between 4.6 to 7.4, when you added the chemicals to the water, they may have been in different amounts changing the PH to a different amount. This could also be the function ...
by Vmedvil
Wed Sep 27, 2017 7:38 am
Forum: Bioinformatics
Topic: query on epitopes of receptors
Replies: 1
Views: 1894

Well, I can do this by visual analysis, but I would start with Antibodies that attach to those receptors and yes they can attach to receptors as well as antigens. Antigens are a type of receptor on Viruses and Bacteria, as i discovered in another post, the "Glycoproteins or antigens for Viruses...
by Vmedvil
Wed Sep 27, 2017 7:08 am
Forum: Molecular Biology
Topic: scientist and Lab for a private project
Replies: 1
Views: 2441

Well, There are problems with this nothing can survive without Energy for too long and plants use photosynthesis which requires water and Carbon dioxide, there is no known way to keep something alive without energy, so a pro tip if you want to keep a plant alive without it being rooted in the ground...
by Vmedvil
Wed Sep 27, 2017 6:35 am
Forum: Molecular Biology
Topic: epitopes
Replies: 1
Views: 1850

Well, an Epitope is the part of a receptor or antigen that a antibody attaches to, so I would start by finding a ribbon diagram of the receptors then remember that it is a part that antibodies attach to, I have never used the Pymol software that you are using so, I would guess look to where a antibo...
by Vmedvil
Wed Sep 27, 2017 6:03 am
Forum: Microbiology
Topic: Question about Viral Glycoproteins
Replies: 1
Views: 1467

I have since this post answered my own question via research into Hybrid vectors, Adenoviruses have molecular holes being more crystalline as viruses like it and other viruses have a lipid envelope such as HIV-1, both are true depending on the species.
by Vmedvil
Wed Sep 27, 2017 5:24 am
Forum: Microbiology
Topic: Can I make lentivirus with pLXSN retrovirus plasmid?
Replies: 1
Views: 1402

No, you cannot make a functioning Retroviral/Lentiviral Vector(Which are actually the same Retroviruses are a class of Lentiviruses). You have problems kinda the pLXSN Plasmid is missing the Pol gene only having Gag, Both Pol and Gag are required for a functioning Vector, but it could be used as a L...
by Vmedvil
Wed Sep 27, 2017 4:54 am
Forum: Genetics
Topic: Mutations P53
Replies: 1
Views: 1717

Well, P53 measures the level of DNA damage and other related factors in the cell and can cause cell death if the damage is bad enough, if the damage is not bad enough the P53 Protein could rather cause the cell to stop reproducing then death. The P53 protein has many functions and connects into many...
by Vmedvil
Tue Aug 15, 2017 10:47 am
Forum: Microbiology
Topic: Growth curves question
Replies: 1
Views: 1986

Well, I would use calculus equations, like this bacteria growth model, you can find the amount of bacteria or cells at any point to any point as long as you know the growth rate of the bacteria using the exponential decay/growth model, just know the number of bacteria or cells at any point, just ins...
by Vmedvil
Tue Aug 15, 2017 10:23 am
Forum: Genetics
Topic: Why would a scientist want "stable overexpressing cells"?
Replies: 1
Views: 2048

Well, Over Expression is the act of adding or changing the promoter at some point that makes the RNA Polymerase generate more mRNA to the Ribosomes to be converted into proteins, This does a very different job than making a new phenotype, this regulates how many copies that this gene is sent to the ...
by Vmedvil
Tue Aug 15, 2017 10:00 am
Forum: Genetics
Topic: Salmon testis DNA and qPCR
Replies: 1
Views: 2532

Well, PCR is a technique which using a DNA polymerase synthetically makes copies of the DNA. The Salmon Testis DNA is added as the DNA to be made copies of by the DNA polymerase in the method of PCR and at a certain temperature the DNA Polymerase will make copies of the DNA that is within the test t...
by Vmedvil
Tue Aug 15, 2017 9:14 am
Forum: Molecular Biology
Topic: What it the relationship between decibel and human insulin?
Replies: 1
Views: 3081

Re: What it the relationship between decibel and human insul

I am not going to point out the many things scientifically wrong with this post but there is no relationship between sound and insulin, it does produce sound when it vibrates just like any molecule but this has no effect on the body and it is quite weak, This poster that you got this information fro...
by Vmedvil
Tue Aug 15, 2017 8:45 am
Forum: Molecular Biology
Topic: Need Help! 3-d atomic model of Mitosis Promoting Factor
Replies: 1
Views: 3089

Get the program YASARA then model it yourself and you are not looking for an atomic structure that is like Protons and Neutrons within a atom along with electrons, you are looking for molecular structure of the protein in amino acids. Get the protein code then plug it into YASARA. The Protein code c...