Search found 6 matches

by SRR
Tue Apr 03, 2018 2:22 pm
Forum: Microbiology
Topic: Can bacteria grow on MES as a carbon source?
Replies: 1
Views: 1987

Can bacteria grow on MES as a carbon source?

Hi All, I have recently made up AF6 culture medium with MES (2-Morpholinoethanesulfonic Acid). The culture became slightly turbid overnight. I have previosly tried a very similar medium with tricine instead of MES, but that didn't become turbid overnight. I have done other experiments where I added ...
by SRR
Thu Jan 18, 2018 2:17 pm
Forum: Microbiology
Topic: Why does Pfennig's medium contain potassium chloride?
Replies: 0
Views: 3911

Why does Pfennig's medium contain potassium chloride?

Pfennig's medium contains ammonium chloride, potassium phosphate and potassium chloride amongst other things. I have seen other culture media which also contain these things and there is a note on one which says that "potassium chloride provides essential ions". Why are these additional ions needed ...
by SRR
Sun Sep 17, 2017 3:36 pm
Forum: Microbiology
Topic: Why doesn't cow manure promote the growth of water mould?
Replies: 1
Views: 1845

Why doesn't cow manure promote the growth of water mould?

I have noticed that when cow manure is added to a pond, the water goes green or green/brown suggesting the growth of organisms such as cyanobacteria, chrysophytes, cryptophytes and euglenoids, it doesn't look like water mould. I find this strange becuase if I add the ingredients of cow manure (ammon...
by SRR
Mon Jul 17, 2017 7:45 pm
Forum: Microbiology
Topic: Clear in the morning, brown in the afternoon
Replies: 0
Views: 1006

Clear in the morning, brown in the afternoon

If I have some water collected from a freshwater source which is clear in the morning, but goes brown later in they day (then repeats the following day), what organisms would cause that? I have read that diatoms can produce this effect, because they divide in unison at night, but are there any other...
by SRR
Mon Jul 17, 2017 7:35 pm
Forum: Microbiology
Topic: Trouble growing Microcystis
Replies: 1
Views: 1424

Re: Trouble growing Microcystis

I found some guidelines by Christopher Bolch on researchgate which solved my problem. My mistake was mainly pH. I was trying to culture an organism from an acidic habitat under alkaline conditions. That was because I had read that if water is green and has white froth on it, then it is a cyanobacter...
by SRR
Tue Apr 18, 2017 12:21 pm
Forum: Microbiology
Topic: Trouble growing Microcystis
Replies: 1
Views: 1424

Trouble growing Microcystis

Hello all, I've been trying to grow the cyanobacterium Microcystis in liquid culture for years. I once found water in a stream in the autumn which had a really strong green/blue/brown tint, in fact a bottle of it looked almost black in certain lighting. There was also a lot of white froth on the wat...