Search found 3 matches

by lch8934402
Wed Apr 08, 2015 2:38 am
Forum: Cell Biology
Topic: how to cultrue IEC-6
Replies: 4
Views: 19965

Re: how to cultrue IEC-6

Normally DMEM medium is modified with 4mM L-glutamin,is that necessary to add 2mM L-glutamin into medium,and what kind of insulin should i choose,you know there are lots kinds of insulin in hospital.
by lch8934402
Fri Mar 27, 2015 9:39 am
Forum: Cell Biology
Topic: how to cultrue IEC-6
Replies: 4
Views: 19965

Re: how to cultrue IEC-6

Hi, Sigma-Aldrich says: DMEM + 2mM Glutamine + 0.1 IU/ml Insulin + 5% Foetal Bovine Serum (FBS); 5% CO2 Different people use different protocols for different experiments. Sometimes they grow fine in either, but change slightly which makes one version more suitable for your experiments. If you don'...
by lch8934402
Thu Mar 26, 2015 4:05 pm
Forum: Cell Biology
Topic: how to cultrue IEC-6
Replies: 4
Views: 19965

how to cultrue IEC-6

still now,i found that many paper mention 10%FBS+DMEM,1x105 U/liter of penicillin/streptomycin at 37┬░Cin an atmosphere of 10% CO2 and 95% relative humidity. But in some forum ,someone said,1U/ml insulin must be add into the cultrue medium. Does anyone tell me why,and what kind of medium shoud IEC-6 ...