Search found 9 matches

by xiaolong88
Tue Dec 11, 2012 4:58 pm
Forum: Molecular Biology
Topic: qPCR
Replies: 2
Views: 3718

qPCR

Hi, I have transcript (RNA) which I would use for qRT PCR for standard curve-the standard. I should quantitate the transcript (RNA) before reverse transcribe them into cDNA. I wonder I should serially dilute the transcript and perform reverse transcription? Or I should synthesis the cDNA first and o...
by xiaolong88
Fri Oct 15, 2010 5:46 am
Forum: Genetics
Topic: Question about Sequencing
Replies: 4
Views: 3834

Thanks Canalon Yes i did go through the article online include the wiki and books(most trusted). I know that there are a lot of methods used in sequencing. But what about IP-RP-HPLC, ion-pair reversed-phase high-performance liquid chromatography. Does this method can used to read short fragment of D...
by xiaolong88
Thu Oct 14, 2010 5:57 pm
Forum: Genetics
Topic: Question about Sequencing
Replies: 4
Views: 3834

Thanks for your info. Yes i did read an article about it but yet i can't find any book which explain about this method. Anyway thanks for your reply
by xiaolong88
Thu Oct 14, 2010 4:39 pm
Forum: Genetics
Topic: Question about Sequencing
Replies: 4
Views: 3834

Question about Sequencing

Honestly there are a lot of DNA sequencing technique, but what is the method used to sequence or to detect a shorter DNA fragment which is extremely small probably <100bp. As i know Sanger sequencing can read up to >500bp.
Would it be illumina sequencing?
by xiaolong88
Sun Jun 13, 2010 1:25 am
Forum: Bioinformatics
Topic: Further study
Replies: 1
Views: 1996

Further study

Can a bioinformatics graduate further study in medical sciences?
Thanks for your comment
by xiaolong88
Tue May 18, 2010 1:46 am
Forum: Biology Movies
Topic: Grey's anatomy
Replies: 2
Views: 11906

Grey's anatomy

Hi guys. Do anyone have the links for this drama series? Grey's anatomy... :wink:
by xiaolong88
Tue May 18, 2010 1:16 am
Forum: Genetics
Topic: E coli replication and transcription
Replies: 6
Views: 6025

Yes^^
by xiaolong88
Sun Oct 11, 2009 3:29 pm
Forum: Bioinformatics
Topic: BLAST
Replies: 9
Views: 32809

Hi Jackbean: Thanks for your reply, well for your information, this is the questions that i need to answer for my exercise by using BLAST, i face difficulty on using it, that why i post questions here to ask for help. The BLAST i use on internet, and i really don't know how to use it. m Thanks a lot...
by xiaolong88
Sun Oct 11, 2009 3:17 am
Forum: Bioinformatics
Topic: BLAST
Replies: 9
Views: 32809

BLAST

I'm sure all bioinformaticians heard of BLAST. Well i'm student of bioinformatics and first time using BLAST. I need help for those who can guide me how to obtain and interprate those information from the result. I did go through the tutorial part of BLAST but i'm not really understand. Hope someone...