Pseudomonas aeruginosa - negative
Reference:
http://www.pubmedcentral.nih.gov/articl ... tid=535267

Pseudomonas fluorescens - negative
Reference
http://aem.asm.org/cgi/reprint/18/3/355.pdf (check Table 1)

Proteus mirabilis - may be positive

Citrobacter freundii - may be negative
Reference
http://jcm.asm.org/cgi/reprint/4/5/453.pdf

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Hi, I am not sure if this is the right explanation, but here is something to think about... At each purificiation step, the number of bands should be decreased. In theory, the protein should be pure when subsequential purification do not lead to any increase in specific activity. So as the number of...

Textbook: iGenetics by Peter J. Russell

Hi,

Just a question: Did you send your sample for sequencing to check if there is any mutation in gene of interest after transformation?

Above mentioned books are really good. I think everyone who wants to pursue their career in research, should have these books. You can also try to find on google book (just write the key words!). Some of them have limited preview, so it will give you more options. I hope this helps!

Use gateway cloning, it's relatively fast. Inhibiting regulatory domain of enzyme can help you in changing catalytic activity (i.e. nonsense mutation may give you gain-of-function)

It is unwise because there are multiple codons exist for one amino acid!

These are basic concepts, you should be able to find online for sure.

Be a Gregor Mendel! Do some pea experiments by yourself.

Form my understanding, polycistronic sites means 1 mRNA is responsible for production of more than one proteins. I know from one experiment that if you have nonsense mutation in lacZ gene, it would result in truncation in lacZ protein and lacY and lacA proteins wouldn't be produce at all.