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Hi, Does anyone have an experience in making a mutant library. I mean introducing radom mutation in a gene and making a library. Also has anyone used XL1 Red strain fron Stratagene?
Hi, I am having issues with Quick change mutagenesis. I am trying to intrduce a single base change in a gene cloned into a plasmid (5Kb). My oligos are 37 bp long with 18 bp homology on either side. I am following the protocol as per the manual but only variation is that a) My oligos are not PAGE pu...