Search found 2 matches

by Charan
Wed Jan 04, 2006 6:51 pm
Forum: Molecular Biology
Topic: Random mutagenesis
Replies: 0
Views: 1108

Random mutagenesis

Hi, Does anyone have an experience in making a mutant library. I mean introducing radom mutation in a gene and making a library. Also has anyone used XL1 Red strain fron Stratagene?
by Charan
Mon Sep 26, 2005 1:48 am
Forum: Molecular Biology
Topic: Quick Change
Replies: 1
Views: 1985

Quick Change

Hi, I am having issues with Quick change mutagenesis. I am trying to intrduce a single base change in a gene cloned into a plasmid (5Kb). My oligos are 37 bp long with 18 bp homology on either side. I am following the protocol as per the manual but only variation is that a) My oligos are not PAGE pu...