Search found 4 matches

by Ilsalmi
Thu Oct 16, 2008 3:09 pm
Forum: Cell Biology
Topic: Mycoplasma and transfection
Replies: 5
Views: 5038

Thank you, wouldn't it be great if there is a 100% reliable test?:) Now I am looking into sending the sample to direct broth culture test and also hoping to find a lab that would do NASBA test for Mycoplasma mRNA - to try to distinguish between residual Myco DNA and actual very low live Myco infecti...
by Ilsalmi
Mon Oct 13, 2008 2:46 pm
Forum: Cell Biology
Topic: Mycoplasma and transfection
Replies: 5
Views: 5038

Mycoplasma and transfection

I have an interresting puzzle: Stably transfected cell line brought to my lab was put in quaratine. Cultured without antibiotics for over 6 weeks, grows great, no obvious problems. Samples were send to a GLP facility for Mycoplasma tests. 3 separate PCR tests came back positive (they do DNA extracti...
by Ilsalmi
Wed May 21, 2008 8:54 pm
Forum: Cell Biology
Topic: Cell Death after selection pressure has been removed?
Replies: 2
Views: 3235

Re: Cell Death after selection pressure has been removed?

Thank you, unfotrunately (fortunately) it is no mystery after all. I have screened the several batches of heat inactivated serum i have been using, and one of them turned out to be just way bad. The same one I used for making up media without ATB. The paralel culture with selection antibiotics was i...
by Ilsalmi
Mon May 19, 2008 2:24 pm
Forum: Cell Biology
Topic: Cell Death after selection pressure has been removed?
Replies: 2
Views: 3235

Cell Death after selection pressure has been removed?

I have an adrenergic receptor stable transfected CHO cells - I have been culturing them in both with and without AB (for quarantine purposes) - and the cell population in media without all antibiotics (incl. the selection ones - G418 and Hygromycin) is dying. There is no bacterial nor Mycoplasmal co...