Search found 10 matches

by biolearner
Mon Dec 29, 2008 9:07 am
Forum: Molecular Biology
Topic: Recombinant DNA techniques
Replies: 0
Views: 2688

Recombinant DNA techniques

I wonder if there are new vectors for recombinant DNA techniques. I prefer a vector integrated with GFP. The below is some web source I bookmarked. The Recombinant DNA technique was engineered by Stanley Norman Cohen and Herbert Boyer in 1973. They published their findings in a 1974 paper entitled &...
by biolearner
Mon Dec 15, 2008 5:49 am
Forum: General Discussion
Topic: Antigen retrieval problem
Replies: 0
Views: 1621

Antigen retrieval problem

Hi, eveyone, I tried to do CD34 membrane antigen retrieval from paraffin embedded tissue section with microwave and enzyme methods, but just found these methods are not working. The protocols I used are as follows. m I used the above methods successfully to retrive nuclear antigen from the same sect...
by biolearner
Thu Nov 27, 2008 9:34 pm
Forum: Molecular Biology
Topic: PCR questions
Replies: 2
Views: 20822

I suggest you the PCR World--everything about polymerase chain reaction (PCR). It's a very organized blog about PCR basis. m Here is the CONTENTS Polymerase Chain Reaction (Molecular Info) History of Polymerase chain reaction (PCR)--(1) History of Polymerase chain reaction (PCR)--(2) Regular PCR Pro...
by biolearner
Fri Oct 10, 2008 5:46 am
Forum: Genetics
Topic: PCR with only one known primer
Replies: 2
Views: 2266

Re: PCR with only one known primer

Your problem is not difficult to solve. Do you know the name of gene? I suggest you visit the PCR Core at:
http://www.pcrworld.blogspot.com/

The anser should be over a paper there.
by biolearner
Fri Oct 10, 2008 5:40 am
Forum: Molecular Biology
Topic: Does age affect dna?
Replies: 3
Views: 3087

Re: Does age affect dna?

Yes, I think so. You can refer the information at DNA World. http://www.u-dna.blogspot.com

I have read a review paper over there.
by biolearner
Fri Jun 20, 2008 5:30 am
Forum: PDF & Doc Files
Topic: Free Books.
Replies: 48
Views: 110476

Re: Free Books.

Here is a super textbook of biochemistry with 24 chapters and 500 units.

http://www.e-biotek.com/ebiotek_321.htm
by biolearner
Mon May 19, 2008 10:07 pm
Forum: Cell Biology
Topic: How can I isolate adult stem cells from circulation.
Replies: 1
Views: 9669

How can I isolate adult stem cells from circulation.

Adult stem cells are very important to design a new diagnosis and develop a new therapy to some degenerative diseases. I wonder how I can isolate adult stem cells from circulation. Is there a specific I can use for the extraction. Below article is an introduction to the definition of adult stem cell...
by biolearner
Mon May 12, 2008 8:31 pm
Forum: Cell Biology
Topic: Looking for: Protocol to Culture HESCs without Feeder
Replies: 0
Views: 1307

Looking for: Protocol to Culture HESCs without Feeder

I have been doing experiments with HESCs, H1 and H9 (from national stem cell bank). Right now, I am co-culturing the cell lines with feeder cell line MEF (from ATCC). I wonder if anyone has the experience to culture H1 and/or H9 without using feeder layer. You are very appreciated if you can let me ...
by biolearner
Mon May 05, 2008 5:52 pm
Forum: Molecular Biology
Topic: DNA Protein Interactions Problems
Replies: 2
Views: 1743

Re: DNA Protein Interactions Problems

Thank you for your tips. I had the exact problem as you mentioned. I try to probe the interactions between the gene PPAR-gamma and the antigen beta-catenin. I did not have a lot of experience with other method to probe the interactions, but I have to find a protocol that can give me a repeatable res...
by biolearner
Sun May 04, 2008 7:47 pm
Forum: Molecular Biology
Topic: DNA Protein Interactions Problems
Replies: 2
Views: 1743

DNA Protein Interactions Problems

I have done a lot of DNA protein interaction assay by gel mobility shift assay (GMSA) for my project, but I just found the results were not repeatable. There is a big difference with the same experimental procedure with the same sample and reagents. I wonder if other methods are better. Filter bindi...