Search found 9 matches

by mmustafa
Wed Apr 16, 2008 8:15 pm
Forum: Molecular Biology
Topic: Primer Design
Replies: 1
Views: 1197

It's better to design them separately. Mismatches will result in amplification of something other than what you have in mind.
by mmustafa
Thu Feb 28, 2008 3:15 pm
Forum: Molecular Biology
Topic: Transformation troubles
Replies: 12
Views: 4023

I got really nice strong single band (correct size) when I ran my PCR product on a gel. That's why I was confident that the transformation would go smoothly!!
by mmustafa
Wed Feb 27, 2008 3:32 pm
Forum: Molecular Biology
Topic: Transformation troubles
Replies: 12
Views: 4023

I got new stock of competent cells and performed transformation using a pUC19 control plasmid and got really tiny colonies. What does that mean??
by mmustafa
Wed Feb 27, 2008 3:09 pm
Forum: Molecular Biology
Topic: Competent cells
Replies: 3
Views: 1889

Can't I buy it from somewhere?
by mmustafa
Wed Feb 27, 2008 1:33 am
Forum: Molecular Biology
Topic: Competent cells
Replies: 3
Views: 1889

Competent cells

Can someone tell me where I can get DH5alpha stock from to make my own competent cells?
by mmustafa
Wed Feb 27, 2008 1:14 am
Forum: Molecular Biology
Topic: Transformation troubles
Replies: 12
Views: 4023

Re: Transformation troubles

Nope! Nothing's different!!!!
by mmustafa
Wed Feb 27, 2008 12:48 am
Forum: Molecular Biology
Topic: Transformation troubles
Replies: 12
Views: 4023

Re: Transformation troubles

I have been heat shocking for 30 to 45 sec at 42C. That should be okay, right?
by mmustafa
Tue Feb 26, 2008 9:58 pm
Forum: Molecular Biology
Topic: Transformation troubles
Replies: 12
Views: 4023

Re: Transformation troubles

I used the control plasmid provided with the competent cells. No colonies there either!!
by mmustafa
Tue Feb 26, 2008 9:05 pm
Forum: Molecular Biology
Topic: Transformation troubles
Replies: 12
Views: 4023

Transformation troubles

Hi All!!

I am struggling with trying to get my plasmid into competent cells. I am currently using the Invitrogen Gateway Directional TOPO kits and One Shot TOP 10 competent cells and have had no success with the transformation process. Any clues anyone?????