Search found 4 matches

by aureliegineste
Mon Feb 15, 2016 10:57 am
Forum: Genetics
Topic: Hepatocyte Nuclear Factor HNF4a
Replies: 1
Views: 3731

Hepatocyte Nuclear Factor HNF4a

Good morning everybody, I am currently studying the transcriptional regulation of a gene in the liver, known to be a target of HNF4α (this gene is strongly upregulated in HNF4α liver specific KO mice) in an inflammatory context (LPS). My hypothesis is that, during inflammation, HNF4α is phosphorylat...
by aureliegineste
Wed Nov 20, 2013 10:26 am
Forum: Cell Biology
Topic: FACS Cells Markers
Replies: 0
Views: 2128

FACS Cells Markers

Hi everyone, I am looking for some informations about immune cells markers in mouse. I founded some markers, but I'm not sure if they are ok. So can you tell me what do you think about my selection ? LT : CD3 LB : CD19 Neutrophiles (most important cells for me) : Ly6G high and ? Dendritic cells : CD...
by aureliegineste
Wed Oct 30, 2013 8:59 am
Forum: General Discussion
Topic: Get neutrophils from mouse liver and spleen
Replies: 0
Views: 1225

Get neutrophils from mouse liver and spleen

Hi everyone,
I am looking for a process to recover neutrophils from mouse tissues (liver and spleen). Is someone have some advices or a protocol ?
Thank you !
Aurélie
by aureliegineste
Wed Sep 04, 2013 7:48 am
Forum: Molecular Biology
Topic: Overexpression
Replies: 2
Views: 5450

Overexpression

Hi everyone ! I need some help. I would like to overexpress a gene (zfp36) in bone marrow derived-macrophages culture (BMDM). I started my PhD few days ago and I don't have a lot of informations about this technical procedure. So I ask you some questions: - Do you have some advice about the vector p...