Search found 440 matches

by jonmoulton
Tue Feb 16, 2016 5:06 pm
Forum: General Discussion
Topic: How does a Virus survive
Replies: 3
Views: 2180

Re: How does a Virus survive

The reservoir of infective viruses is usually within hosts, though the virus can remain infective in the environment for a while. Influenza can replicate in several species, including humans, chickens and pigs. It is usually transmitted between people by aerosol droplets expelled by an infected orga...
by jonmoulton
Wed Nov 18, 2015 4:21 pm
Forum: Molecular Biology
Topic: Why is TSH reported in mIU/l
Replies: 4
Views: 2944

Re: Why is TSH reported in mIU/l

If you have an easily denaturable protein or peptide (which I'll call "stuff"), different preparations will have different ratios of active (good) stuff to non-functional (crappy) stuff. If you define your experiments in terms of mg of the stuff, you will end up with no consistency of the ...
by jonmoulton
Tue Jul 21, 2015 6:18 pm
Forum: Genetics
Topic: Knockdown vs Knockout
Replies: 3
Views: 7725

Re: Knockdown vs Knockout

Here is some very recent information about knockdowns versus knockouts. Genetic compensation, as adjustments in gene expression to ease the impact of a deleterious mutation, are observed by this group in knockout animals but not knockdown animals. Rossi A, Kontarakis Z, Gerri C, Nolte H, Hölper S, K...
by jonmoulton
Mon Jul 13, 2015 4:29 pm
Forum: Molecular Biology
Topic: Mutants and knockdowns differ in compensation response
Replies: 1
Views: 2303

Mutants and knockdowns differ in compensation response

If you want to knock down a gene to learn about its function, it can be useful to assess the loss of function without a background of altered gene expression due to compensation. Now there are data showing that mutants can compensate and the response differs from that of morphants. Rossi A, Kontarak...
by jonmoulton
Mon Jun 15, 2015 2:48 pm
Forum: Genetics
Topic: Knockdown vs Knockout
Replies: 3
Views: 7725

Re: Knockdown vs Knockout

There are many considerations. Here are a few. For a one-time heritable change that will yield a model organism line you can use for years, go with a knockout. If you are targeting an embryonic lethal gene, you will probably need to use a knockdown. You can either use a reduced dose of the knockdown...
by jonmoulton
Mon Feb 23, 2015 3:50 pm
Forum: Molecular Biology
Topic: How to read this tricky electropherogram?
Replies: 1
Views: 5327

Re: How to read this tricky electropherogram?

Do you know if this is genomic DNA or cDNA? If not, you should BLAST against both the RNA sequences and the genomic DNA sequence.
by jonmoulton
Mon Dec 29, 2014 11:01 pm
Forum: General Discussion
Topic: How much math do you need to know in order to do biology?
Replies: 6
Views: 13421

Re: How much math do you need to know in order to do biology?

What are you trying to find out, Alison?
by jonmoulton
Mon Dec 15, 2014 5:33 pm
Forum: General Discussion
Topic: Beginner Microbiologist, looking for a place to start.
Replies: 1
Views: 3779

Re: Beginner Microbiologist, looking for a place to start.

If you are seriously interested in microbiology, you need to delve early into chemistry: general chem, organic chem, biochem. Finding some used college texts is a good way in, but it can be expensive. You can get a lot from Google and Wikipedia. I'm going to assume you are smart and highly motivated...
by jonmoulton
Thu Nov 06, 2014 4:42 pm
Forum: Microbiology
Topic: Ebola 2014: Medical & Ethical Issues (youtube, lecture)
Replies: 0
Views: 2435

Ebola 2014: Medical & Ethical Issues (youtube, lecture)

Ebola 2014: Medical & Ethical Issues by Dr. Pat Iversen at Oregon State University in Corvallis, OR, USA. Lecture on 4 Nov 2014. https://www.youtube.com/watch?v=Nd4gSMDCG5U
by jonmoulton
Wed Oct 29, 2014 3:58 pm
Forum: Genetics
Topic: Exp. antisense treatment for Pompe disease
Replies: 0
Views: 3197

Exp. antisense treatment for Pompe disease

Here's a nice preclinical report of antisense as a potential therapeutic. Antisense Oligonucleotide-mediated Suppression of Muscle Glycogen Synthase 1 Synthesis as an Approach for Substrate Reduction Therapy of Pompe Disease. Clayton NP, Nelson CA, Weeden T, Taylor KM, Moreland RJ, Scheule RK, Phill...
by jonmoulton
Wed Oct 08, 2014 4:40 pm
Forum: General Discussion
Topic: Computer Science + Ecology/Ethology/Conservation biology = ?
Replies: 4
Views: 3031

Re: Computer Science + Ecology/Ethology/Conservation biology = ?

I can offer a taste of the direction you are discussing, as well as a specific tool which you might find exciting. Look into HexSim:
http://www.hexsim.net/