Search found 3 matches

by Irene76
Thu Dec 13, 2007 10:21 am
Forum: Molecular Biology
Topic: Storage of Blotted nitrocellulose membrane
Replies: 3
Views: 4249

Hi, normally i store the blotted membrane (i use PVDF) after a wash in TBST, on 4°C wrapped in a transparent membrane. I used some filters also after a year!
by Irene76
Mon Dec 10, 2007 5:34 pm
Forum: Molecular Biology
Topic: N-Cadherin western blotting
Replies: 2
Views: 2012

Thank you for your advices, but unfortunatly I don't have a cell line of neurological origin because I work with normal human keratinocytes that I have infected with a gene responsable of EMT. The only cell lines that I have are the ones that I have tested.
Regards
by Irene76
Sun Dec 09, 2007 11:16 am
Forum: Molecular Biology
Topic: N-Cadherin western blotting
Replies: 2
Views: 2012

N-Cadherin western blotting

Hi, I need to perform a western blotting using a N-Cadherin antibody. I used the antibody from BD Bioscences on hela, huvec and human fibroblasts, hoping these cells were good positive controls but I had no results. Do You know if the matter is caused by bad antibody supplied from BD or bad cells as...