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by cauguama
Wed Aug 03, 2005 6:56 pm
Forum: Molecular Biology
Topic: Plasmid PCR
Replies: 2
Views: 3554

When you run the PCR reaction, your plasmid DNA will denature to ssDNA and be available for binding to your primers. So turning it into linear DNA is not required. When figuring your temperatures for the reactions, it helps to take into account the sequence of your primers so as to know the correct ...