Search found 27 matches

by h2so4hurts
Sat Apr 05, 2008 5:58 pm
Forum: General Discussion
Topic: Biology Teacher
Replies: 6
Views: 2960

Too late?!?! Haha, i know people that started to get their teaching degree when they were 30. You'll be fine. Good luck!
by h2so4hurts
Thu Apr 03, 2008 8:04 pm
Forum: Molecular Biology
Topic: PCR primer design
Replies: 2
Views: 1148

1. depends what you're using it for. You won't get the whole gene if the 3' primer isn't at the end or outside of the coding sequence 2. It could be a true gene or a pseudo gene. BLAST the terminal exon. It was found because it has coding sequence followed by a polyA track. Most of the predicted gen...
by h2so4hurts
Wed Apr 02, 2008 11:58 pm
Forum: Molecular Biology
Topic: m RNA purification
Replies: 1
Views: 988

Trizol extraction (Or phenol chloroform) and then an ethanol precipitation.

See:
http://www.labspaces.net/protocols.php? ... 0isolation
by h2so4hurts
Wed Feb 27, 2008 9:17 pm
Forum: Molecular Biology
Topic: Transformation troubles
Replies: 12
Views: 4049

Haha, or he didn't let the plates grow long enough...

Also, try adding 4uL of your PCR product into to TOPO reaction, especially if your PCR product is weak. Maybe try gel purifying your PCR product before the TOPO reaction.
by h2so4hurts
Tue Feb 26, 2008 3:14 pm
Forum: Cell Biology
Topic: outer edge of cells
Replies: 4
Views: 2849

Haha, I love these questions. Look at a picture...
by h2so4hurts
Tue Feb 26, 2008 3:00 pm
Forum: Molecular Biology
Topic: unspecific co-immunoprecipitation
Replies: 1
Views: 1064

Re: unspecific co-immunoprecipitation

I think we're going to need some details to help you out on that one. Is this a control experiment?
by h2so4hurts
Mon Feb 25, 2008 9:29 pm
Forum: Molecular Biology
Topic: DNA sequencing
Replies: 3
Views: 1575

CG 3 H bonds
AT 2 h bonds

AT is easier to break apart, but if the reaction is hot enough that point is moot :P
by h2so4hurts
Mon Nov 26, 2007 5:05 pm
Forum: Molecular Biology
Topic: Can you reuse qiashredder columns?
Replies: 3
Views: 3488

All the qiashredder columns do is tear open your tissue, they shouldn't bind nucleic acid. I don't see why you can't just was them really well, or just pull your sample through a 22guage or smaller syringe.
by h2so4hurts
Mon Nov 26, 2007 5:01 pm
Forum: Bioinformatics
Topic: NEED HELP!
Replies: 4
Views: 4325

This sounds like a homework question. I'm pretty sure there are easy tests to determine which major operons regulate your gene... Its probably in your book. Changing the mechanism is pretty easy, just change the operator sequence upstream of the gene. I'm not really sure what the limitations of your...
by h2so4hurts
Wed Nov 21, 2007 4:57 pm
Forum: General Discussion
Topic: Biology Hangman Game
Replies: 582
Views: 273267

Hahaha, Woolleyy wins
by h2so4hurts
Tue Nov 20, 2007 7:04 pm
Forum: Ecology
Topic: Island natural selection
Replies: 2
Views: 2457

I posted a story about this a few weeks ago from science daily.
http://www.labspaces.net/view_link.php?newsID=547
by h2so4hurts
Tue Nov 20, 2007 6:57 pm
Forum: Botany Discussion
Topic: quantosome
Replies: 4
Views: 3425

He'd probably answer his own question by responding to yours :P