Search found 12 matches

by benedictine
Sat Dec 08, 2007 2:07 pm
Forum: Molecular Biology
Topic: DNA extraction from dried blood specimen
Replies: 0
Views: 966

DNA extraction from dried blood specimen

hi, I have done pcr on DNA extracted from archive dried blood specimen (filter paper) stored at room temperature; most of the amplification did not work. DNA was extracted using TE-method, add 65ul TE buffer, 50 deg for 15 min, then 97 deg 15 min, centrifuge 2-3 sec. The problem is not with the PCR ...
by benedictine
Mon Nov 26, 2007 11:01 pm
Forum: Molecular Biology
Topic: Digest
Replies: 7
Views: 3107

thanks for the link
from bene
by benedictine
Mon Nov 26, 2007 10:59 pm
Forum: Molecular Biology
Topic: acrylamide question
Replies: 5
Views: 3368

thanks

to canalon and blr11 thanks for your advice and sorry I am replying late The acrylamide was in a glass round "bottle" with a long neck; this is the description so it cannot be reached by spatula or spoon. Also, 2 days ago I couldn't find the bottle; I also dont know who removed it, or what...
by benedictine
Fri Nov 16, 2007 3:53 pm
Forum: Molecular Biology
Topic: Digest
Replies: 7
Views: 3107

blcr11

thanks for writing. It says on the bottle TaqI, not Taqal. Thanks a lot. I use at the most 1ul of enzyme per sample, is that a lot? and from where do we check if our sites are partially methylated? is it from sequence?
thanks again, bene
by benedictine
Fri Nov 16, 2007 3:48 pm
Forum: Molecular Biology
Topic: acrylamide question
Replies: 5
Views: 3368

glass

thank you for your reply The problem is that it is inside a glass "bottle" like an erpenmeiyer (I dont know how it is called in english) but its glass with a round bottom and the top is very long. Kind of like a lab wine-bottle. I dont know the exact name. that means we can never use this ...
by benedictine
Thu Nov 15, 2007 2:18 pm
Forum: Molecular Biology
Topic: acrylamide question
Replies: 5
Views: 3368

acrylamide question

Dear friends
can someone please advise me how to remove acrylamide that has solidified (left in beaker left in refrigerator)?

thank you
from bene
by benedictine
Thu Nov 15, 2007 2:17 pm
Forum: Molecular Biology
Topic: Digest
Replies: 7
Views: 3107

to pluto

sorry I dont know what is star activity. What is that? All I know is it is supposed to cut the genes of alleles of a CYP2 that I'm studying. thanks, bene. also, do you have any idea what to do with acrylamide that has solidified in the beaker (in the refrigerator)? How can we remove it? (I will post...
by benedictine
Mon Nov 12, 2007 11:32 am
Forum: Molecular Biology
Topic: Help with reagents used in RNA estraction procedure
Replies: 1
Views: 1141

hi, actually if u get the Sambrook & Maniatis lab manuals, now I cant remember which volume, comes in 3 volumes, u read that and can find answer there
by benedictine
Sun Nov 11, 2007 8:15 pm
Forum: Molecular Biology
Topic: Digest
Replies: 7
Views: 3107

Digest

hi please may I ask; I did a digest using TaqI; its supposed to have 1,2 or 3 bands according to the polymorphism, but why am I getting sometimes 4 bands and 5. What happened and what should I do to avoid this.
Thank you, from bene
by benedictine
Fri Nov 09, 2007 9:48 pm
Forum: General Discussion
Topic: conversion confusion
Replies: 2
Views: 7499

to blcr11

thanks a lot!
from bene
by benedictine
Thu Nov 08, 2007 10:52 pm
Forum: General Discussion
Topic: conversion confusion
Replies: 2
Views: 7499

conversion confusion

hi, can someone guide me, how do I convert say x ug/ul of stock solution to 25 pmol/ul working solution?
First with x ug/ul; I use the molecular weight to calculate how many moles in that stock solution. After that what do I do to get to the pmol/ul? I am confused with the pmol/ul part.
thank you.
by benedictine
Thu Nov 08, 2007 10:47 pm
Forum: General Discussion
Topic: Help!!
Replies: 3
Views: 1542

by the recycle materials perhaps they mean, for example the way the carbon goes; the pathways