Search found 2 matches

by chinna
Wed Aug 03, 2005 5:37 pm
Forum: Molecular Biology
Topic: Plasmid PCR
Replies: 2
Views: 3579

Plasmid PCR

If i am having gene of interest present on Plasmid. And i want to do PCR of that particular gene. How shud i proceed for PCR??? I mean do we hav to get Plasmid linearized prior to PCR or it can work with normal cycles of PCR having profile of Denaturation,annealing and extension.
by chinna
Sun Jul 17, 2005 5:00 pm
Forum: Molecular Biology
Topic: Leishmania PCR
Replies: 0
Views: 1709

Leishmania PCR

hi!! i m working on Leishmania donovani's GP 63 protein. Its a surface protein and helps in virulence, basically help parasites to get entered in macrophages. I am using primers for this protein. I tried a lot of combinations of different PCR parameters. But i did not get bands. The GC contents of p...