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If i am having gene of interest present on Plasmid. And i want to do PCR of that particular gene. How shud i proceed for PCR??? I mean do we hav to get Plasmid linearized prior to PCR or it can work with normal cycles of PCR having profile of Denaturation,annealing and extension.
hi!! i m working on Leishmania donovani's GP 63 protein. Its a surface protein and helps in virulence, basically help parasites to get entered in macrophages. I am using primers for this protein. I tried a lot of combinations of different PCR parameters. But i did not get bands. The GC contents of p...