Search found 26 matches

by hanhan2008
Fri Apr 25, 2008 6:53 pm
Forum: Molecular Biology
Topic: ELISA question
Replies: 1
Views: 1681

Detailed questions: 1. Could ELISA kits of different batches make significant differences? 2. Which company provides the best ELISA kits, or MIP-2 kits in particular? Or does the company matter? 3. If you always do ELISA the same way with little variations in incubation times and such, is it true th...
by hanhan2008
Fri Apr 25, 2008 5:02 pm
Forum: Molecular Biology
Topic: ELISA question
Replies: 1
Views: 1681

ELISA question

I'm doing MIP-2 ELISA for a cell line using a kit from Biosource (now part of Invitrogen). Reproducibility is bad in terms of the equations obtained from the standard curves and the values obtained between different experiments. The big intercepts (big blank readings) caused big variations in calcul...
by hanhan2008
Mon Mar 10, 2008 7:43 pm
Forum: General Discussion
Topic: Relationship of inflammation & oxidative stress
Replies: 0
Views: 882

Relationship of inflammation & oxidative stress

Hi Guys, I know the differences btw the 2 concepts and I also know roughly that they are usually not independent. Could anybody here enlighten me on the details of their inter-dependance? Thanks.
by hanhan2008
Mon Mar 10, 2008 7:31 pm
Forum: General Discussion
Topic: Primary cells vs. cell line: which is more resistant?
Replies: 4
Views: 3695

Thanks a lot, guys! One frequent criticism from in vivo people on cell culture studies using lung epithelial cell lines is the comparability or generability of in vitro results to in vivo situation since in many cases, it takes unrealistically high doses to cause a measurable effect in cultured cell...
by hanhan2008
Tue Mar 04, 2008 9:31 pm
Forum: Molecular Biology
Topic: An ROS producer that doesn't lead to protein carbonylation
Replies: 0
Views: 1580

An ROS producer that doesn't lead to protein carbonylation

Recently, I found that one of my nanoparticles (silver) could not lead to elevated protein carbonyl levels (by DNP method using ELISA) in cultured cells, though in ROS studies, it does give a positive result. Any explanation? Thanks!
by hanhan2008
Tue Mar 04, 2008 8:05 pm
Forum: General Discussion
Topic: Primary cells vs. cell line: which is more resistant?
Replies: 4
Views: 3695

Primary cells vs. cell line: which is more resistant?

Which is generally more resistant to toxicants?
1. Stem cells vs. differentiated cells.
2. Primary cells vs. cell line
3. Immortalized cell line vs. non-immortalized cell line.
It'll be great if somebody could give some links to some research papers on these topics.
Thanks!
by hanhan2008
Tue Mar 04, 2008 7:53 pm
Forum: General Discussion
Topic: Is There A Living Thing With NO CELLS?
Replies: 33
Views: 22406

Re: Is There A Living Thing With NO CELLS?

Virus IS a good answer. Maybe prion is another answer.
by hanhan2008
Wed Feb 20, 2008 11:39 pm
Forum: Molecular Biology
Topic: Can Western blot be completely replaced by ELISA?
Replies: 2
Views: 2227

Can Western blot be completely replaced by ELISA?

Sorry for this naive question. I've been wondering what are the advantages and disadvantages of Western blotting compared to ELISA. Thanks in advance!
by hanhan2008
Thu Dec 27, 2007 8:37 pm
Forum: Evolution
Topic: What a role does NaCl supplement play in human evolution?
Replies: 7
Views: 4426

Re: What a role does NaCl supplement play in human evolution?

That's a good point! What about cooked food in IQ improvement (again, on evolutionary time scale )?
by hanhan2008
Mon Dec 24, 2007 9:42 pm
Forum: Evolution
Topic: What a role does NaCl supplement play in human evolution?
Replies: 7
Views: 4426

Re: What a role does NaCl supplement play in human evolution?

Thanks. Just wonder if it's a possibility (if not convincing) why we human beings are smarter than other animals. Only humans take table salts intentionally while other animals don't.
by hanhan2008
Mon Dec 24, 2007 2:44 am
Forum: Zoology Discussion
Topic: Is there a cell line that can synthesize ascorbic acid?
Replies: 3
Views: 3107

Is there a cell line that can synthesize ascorbic acid?

Just learned that many mammals (rat, mouse, etc.) can synthesize Vit. C in the liver for themselves. I has not even got a chance to check which cell type does the job (probably hepatocyte?).
by hanhan2008
Mon Dec 24, 2007 2:33 am
Forum: Evolution
Topic: What a role does NaCl supplement play in human evolution?
Replies: 7
Views: 4426

What a role does NaCl supplement play in human evolution?

Not sure if somebody else has sparked such a topic. I wonder how convincing to state that NaCl, among other things, contribute to our (apparently) higher IQs compared with other organisms. When think about this, don't forget that excessive NaCl intake contributes to increased occurrence of cardiovas...