Search found 15 matches

by simina
Sat May 11, 2013 11:09 am
Forum: Cell Biology
Topic: Nuclear editing
Replies: 0
Views: 1278

Nuclear editing

Does anyone know why is it advantageous for nuclear processing to occur co-transcriptionally?
by simina
Wed Mar 20, 2013 11:13 am
Forum: Molecular Biology
Topic: RNA
Replies: 6
Views: 23390

I really do not understand the difference between the cis-acting sequences and trans-acting sequence. I read many definitions but is still not clear for me. Any idea of simple and easy explanation that I could understand?:D
by simina
Tue Mar 19, 2013 10:42 am
Forum: Molecular Biology
Topic: RNA
Replies: 6
Views: 23390

Hi! Can anyone explain me how RNA chain grow and what is the role of CTP and ATP in this process?? thanks!!
by simina
Mon Mar 18, 2013 1:31 pm
Forum: Genetics
Topic: Genetics problems
Replies: 3
Views: 6926

Hii!!! I was wandering if a phenotype is expressed as a consequence of 2 different genes, are these genes part of an operon or are regulated by different promoters, meaning that can be located far away? ( in a prokaryotic organisms)??
by simina
Sat Mar 09, 2013 7:53 pm
Forum: Molecular Biology
Topic: Microparticles and flow cytometry
Replies: 5
Views: 3768

Why could be the reason of finding more B cells in the splean in comparison with T cells???
by simina
Sun Mar 03, 2013 10:54 am
Forum: Molecular Biology
Topic: Microparticles and flow cytometry
Replies: 5
Views: 3768

I know in which part of the graph the cells are but I am not sure how to calculate the percentage because I understood that we have to subtract the autoflourescence ones from other graph.. something like that. It is very confusing!!
by simina
Thu Feb 28, 2013 5:43 pm
Forum: Molecular Biology
Topic: Microparticles and flow cytometry
Replies: 5
Views: 3768

I have a question regarding flow cytometry too!!! Please heeelp. I have a lot of questions based on the cytometry pictures, where they ask to calculate percentage of dead cells, or of CD45R etc. Does anyone know how to guide me through the correct answer???
by simina
Mon Feb 25, 2013 6:19 pm
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 24897

great! Thank you very much for your help
by simina
Mon Feb 25, 2013 3:04 pm
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 24897

Is this technique has a special name, for example FRET or you would suggest to give this explanation straight away, without mentioning any name? It is FRET method quite similar to this one? Thank you!1
by simina
Tue Feb 19, 2013 9:24 pm
Forum: Genetics
Topic: Over expressing proteins in mammalian cells
Replies: 2
Views: 2509

Over expressing proteins in mammalian cells

Can anyone tell me an advantage and a disadvantage of over expressing proteins in mammalian cells to discover their function or localisation?
by simina
Tue Feb 19, 2013 9:08 pm
Forum: Molecular Biology
Topic: Difference between ICC and IF
Replies: 7
Views: 24897

I also have some doubts about fluorescence microscopy!? What technique we can use to visualise by fluorescence microscopy a transfected proteins that laks any fluorochrome in mammalian cell cultures? Does anyone have any ideas?
by simina
Fri Feb 15, 2013 2:38 pm
Forum: Genetics
Topic: DNA
Replies: 8
Views: 10166

That is more clear for me thank you. Some bands are indeed a bit bigger than others, although it is not very clear!