Search found 4 matches

by Julian92
Tue Jan 29, 2013 3:10 pm
Forum: Genetics
Topic: PCR
Replies: 2
Views: 5738

I realise that each of the 12 primer pairs bind to a different region of human genome; the VNTR's within each region, and that is how 12 distinct bands can be detected after PCR. But what i don't know is why the exact same primers that target the same location end up a different areas.

Yours,
Julian
by Julian92
Mon Jan 28, 2013 12:25 pm
Forum: Off Topic Discussion
Topic: Which is best? Biology or Chemistry or Physics
Replies: 21
Views: 74969

Re: Which is best? Biology or Chemistry or Physics

Well definitely not physics xD
by Julian92
Mon Jan 28, 2013 12:24 pm
Forum: Off Topic Discussion
Topic: Introduction for members
Replies: 772
Views: 580014

Julian, 20. Studying medicine at the university of glasgow
by Julian92
Mon Jan 28, 2013 12:23 pm
Forum: Genetics
Topic: PCR
Replies: 2
Views: 5738

PCR

Can someone tell me why a mixture of 12 specific primers produces 12 bands from a single persons DNA. And why is the pattern of bands produced by multiplex PCR with the same 12 primers highly variable between individuals? What are the most likely explanations for individual D having less than 12 ban...