Search found 2 matches

by moabubaker
Fri Oct 19, 2012 12:33 pm
Forum: Molecular Biology
Topic: Dilution problem for my Bradfords assay
Replies: 3
Views: 1849

Re: Dilution problem for my Bradfords assay

No, this is one of the standards I used to construct the absorbance/conc curve. I'm just wondering if you can help me figure out how adding 2mg/ml BSA stock with 10ul diluent and 150ul of bradford reagent gives a final BSA conc of 1500ug/ml. I suck at maths and im having a hard time understanding ho...
by moabubaker
Fri Oct 19, 2012 11:55 am
Forum: Molecular Biology
Topic: Dilution problem for my Bradfords assay
Replies: 3
Views: 1849

Dilution problem for my Bradfords assay

Can someone help me understand why a sample has a final concentration of 1500ug/ml. If I add 2mg/ml of stock, 10ul of water and 150ul of bradfords reagent.

If someone can point me in the right direction of how they worked it out. Thanks a lot.