Search found 42 matches

by Helics
Sat Feb 13, 2016 12:13 pm
Forum: Molecular Biology
Topic: Family Specific Primers
Replies: 1
Views: 2017

Family Specific Primers

can any body suggest me how to design gene family specific primers ? Like one gene cluster "ica" have eight different gene as; icaA,icaB,icaC,icaD,icaE,icaF,icaG,icaH Now, how to design a single primer pair for that "ica"cluster ??? Please response as soon as possible. I'm in tro...
by Helics
Thu Jan 28, 2016 8:51 pm
Forum: Molecular Biology
Topic: My Multiplex PCR reaction works, but not my single rxn
Replies: 2
Views: 3903

Hi there.
do you like to provide rxn. mix details of both cases ?
by Helics
Tue Jun 23, 2015 5:45 pm
Forum: Molecular Biology
Topic: Metagenomic ?
Replies: 0
Views: 2276

Metagenomic ?

Please help me.
I want to study unculturable microorganism from soil.
want to clone them
how do I select vector and restriction enzymes ?

Is it possible to culture the in so called laboratory conditions ?

Please help me.
by Helics
Sun Nov 30, 2014 10:00 am
Forum: Microbiology
Topic: Starch Hydrolysis
Replies: 0
Views: 3653

Starch Hydrolysis

Normal RECIPE for STRACH Hydrolysis:

Starch agar composition (g/liter)

Beef extract 3 g

Soluble starch 10 g

Agar 12 g

Distilled water 1 liter

But should we can use Peptone instead of Beef extract ?
by Helics
Mon Aug 11, 2014 9:46 am
Forum: Molecular Biology
Topic: pGP704
Replies: 3
Views: 5618

pGP704

Can any one suggest how do i get plasmid pGP704 ?
by Helics
Sat Aug 02, 2014 3:55 am
Forum: Molecular Biology
Topic: Plasmid yield/concentration
Replies: 3
Views: 5070

Re: Plasmid yield/concentration

Dear, scientist
please give a response.
by Helics
Fri Aug 01, 2014 6:07 pm
Forum: Molecular Biology
Topic: 5' LTR sequece
Replies: 2
Views: 5240

You may try to find comparable restriction endoneucleases for wild type 5'LTR.
Hybrid 5'LTR generates different
size RE digest products.
by Helics
Thu Jul 31, 2014 3:40 pm
Forum: Molecular Biology
Topic: Plasmid yield/concentration
Replies: 3
Views: 5070

Plasmid yield/concentration

What is the difference between high yield plasmid and high concentration plasmid ?
by Helics
Tue Apr 08, 2014 4:26 am
Forum: Molecular Biology
Topic: Restriction Digesion
Replies: 4
Views: 5097

Dear scientist, Jack Bean I need your response.
by Helics
Sun Apr 06, 2014 4:07 pm
Forum: Molecular Biology
Topic: Restriction Digesion
Replies: 4
Views: 5097

Restriction Digesion

which is the best option for Restriction Endonuclease digesion ???

A) 37' C water bath
B) 37' C PCR program
by Helics
Tue Mar 04, 2014 4:33 pm
Forum: Molecular Biology
Topic: Polymorphism and Mutation
Replies: 2
Views: 5300

Polymorphism and Mutation

What is the difference between Gene Polymorphism and Gene Mutation :?: :?: :?:
please give a clear concept. :|
by Helics
Sun Feb 23, 2014 4:59 pm
Forum: Molecular Biology
Topic: Annealing Temparature
Replies: 3
Views: 4821

Re: Annealing Temparature

Primer_F: 5'-GCTCTAGAGTGATAGTGAGCATGG-3'

Primer_R: 5'-GCGATATCCTACACCCAAGCTGC-3'


CORRECTED PRIMER SEQUENCE.

THANKS ALL.
I WILL TRY SUGGESTED EXP. CONDITION.

THANKS "vladCH".