Search found 42 matches

by Katy_Bobbles
Fri Jul 11, 2008 8:25 pm
Forum: Cell Biology
Topic: Stupid and Easy question
Replies: 1
Views: 2247

Re: Stupid and Easy question

The key word is amphipathic ;) Examples are given here
http://www.biology-online.org/dictionary/Amphipathic
by Katy_Bobbles
Thu Jul 10, 2008 2:11 pm
Forum: Molecular Biology
Topic: totalRNA isolation for miRNA
Replies: 2
Views: 3383

Re: totalRNA isolation for miRNA

I can't comment on the sodium acetate but when I did RNA isolation I used isopropanol for the precipitation phase. 0.5ml for every ml of TRI reagent that was used initially. All my 260/280 values were between 1.8 and 2. Your results indicate protein contamination. I isolated RNA from around 12 sampl...
by Katy_Bobbles
Tue Apr 29, 2008 4:53 pm
Forum: Molecular Biology
Topic: COEFFICIENT OF VARIATION :(
Replies: 1
Views: 2083

Re: COEFFICIENT OF VARIATION :(

I was told it should be less than 5. A bit of a difference there but I suppose it may depend on the method used. I used spectrophotometry and the Biuret method to assess QC albumin levels in 20 samples. Hmm :?
by Katy_Bobbles
Tue Apr 29, 2008 4:35 pm
Forum: Human Biology
Topic: Substintia Nigra
Replies: 3
Views: 3784

Neuromelanin is an anti-oxidant. It helps protect the neurones from reactive oxygen species like superoxides and peroxides :)
by Katy_Bobbles
Tue Feb 19, 2008 9:00 pm
Forum: General Discussion
Topic: What is cancer?(in genral)
Replies: 8
Views: 9379

Cancer is described a multitude of cells that grow outwith normal control. This is a way of looking at how cancer occurs. In the cell cycle there are lots of checkpoints or "pit-stops" where the cells are checked to ensure they are growing appropriately. For example there is one between th...
by Katy_Bobbles
Tue Jan 15, 2008 1:40 pm
Forum: Human Biology
Topic: Granuloma
Replies: 3
Views: 3870

Yeah that's right. Don't know about sarcoidosis but I had an exam last week on TB so here is my summary of what causes it. The Mycobacterium tuberculosis rods are engulfed by alveolar macrophages. This kills about 90% of the rods but the remaining 10% evade the host cell's microbicidal killing. They...
by Katy_Bobbles
Thu Jan 10, 2008 2:31 pm
Forum: Microbiology
Topic: chlamidia pneumoniae
Replies: 3
Views: 3084

It is called Chlamydophila pneumoniae now.

Google that and you will find loads!
by Katy_Bobbles
Sun Dec 23, 2007 10:26 am
Forum: Zoology Discussion
Topic: Epilepsy in gerbils.
Replies: 0
Views: 2841

Epilepsy in gerbils.

I'm a people person not a zoology person so any help would be appreciated. Gerbils! Last night my nephew came into my room holding his gerbil. It was just sitting there staring up at me and it's hind leg slightly twitching. Later on it started rolling around an having violent convulsions. This went...
by Katy_Bobbles
Thu Nov 08, 2007 8:59 pm
Forum: General Discussion
Topic: Ham Sandwich?!
Replies: 5
Views: 4790

I would think about what the sun does in an essential step in growth of an important substance in bread. Also the ham...where did it come from (and I don't mean ASDA or Tesco!) Before the shops! Have a think about it. :)
by Katy_Bobbles
Mon Nov 05, 2007 10:25 pm
Forum: Molecular Biology
Topic: RAW 264.7 Macrophages
Replies: 2
Views: 4698

Thanks again bcr11. I just wondered in case it was relevant and I had to write about it. I'm not that desperate to know so I'll just leave it at that. If I have to find out at least I'll know who to write to. :)
by Katy_Bobbles
Mon Nov 05, 2007 2:06 pm
Forum: Molecular Biology
Topic: RAW 264.7 Macrophages
Replies: 2
Views: 4698

RAW 264.7 Macrophages

I've been doing my honours project and would like to know if RAW stands for anything. I know they are murine macrophages but I wondered if the RAW part is relevant??

:)
by Katy_Bobbles
Sun Nov 04, 2007 11:45 am
Forum: Molecular Biology
Topic: about ATPS
Replies: 4
Views: 1781

I assume the ATP S is ATP synthase, the enzyme which catalyses the addition of a phosphate group to ADP to make ATP

Hope that makes sense and it is what the S stood for :)