Search found 4 matches

by FlyMusings
Sat Sep 29, 2012 5:56 pm
Forum: Genetics
Topic: Recombinant DNA not degraded inside transformed cells, why?
Replies: 10
Views: 9033

I think bacteria can take up two plasmids, but taking up plasmids and expressing them is a big toll on the bacteria. So bacteria that took up two plasmids are less healthy than those that took up one plasmid. Over culture, bacteria with one plasmid simply outgrow those with two plasmids.
by FlyMusings
Sat Sep 29, 2012 5:45 pm
Forum: Molecular Biology
Topic: Question regarding about PCR - DNA or RNA as template
Replies: 1
Views: 5828

Do you have controls with the RNA without PCR, run on a gel? I am also a little surprised that PCR can amplify RNA.
by FlyMusings
Sat Sep 29, 2012 5:26 pm
Forum: General Discussion
Topic: how to live and work with animals and forests
Replies: 5
Views: 7114

Just understanding about the finance situation, have you taken quite a bit of debt to finance your medical school eduction? If it is not cheap, you probably cannot just quit the medical profession altogether. I don't know much about medical school, but maybe you do not need the specialization and co...
by FlyMusings
Sat Sep 29, 2012 5:14 pm
Forum: General Discussion
Topic: I have a problem seeing the bigger picture of research
Replies: 2
Views: 3159

It looks like the problem is with figuring out what is the use of your work. DNA sequencing of a species can yield quite a bit of new knowledge. Phylogenetics is one thing, comparison to a more well-studied species to understand how they are different. But the other possible aspect is to study the o...