I want to transfect my cells with a vector for promoter activity analaysis, I need to detect the binding effeciency of some transcription factors to the promoter, what technique should I use? I have read about ChIP but don't know how to design and perform this experiment practically , your help is h...

Hi

I am using Stepone plus (applied biosystem) to perform a gene expression assay using relative quantitation. My query is regarding the output result. There are 4 types of graph output: linear, Log10, Ln, Log2. What is the best graph type to show in my report??

Hi I am wondering if intron have regulatory elements other than splicing recognition signals (Donor, Acceptor and branch point sites). because I have found a mutation in the middle of the intron of my gene and noticed that the patient has some phenotypic resulted from this mutation. So is there any ...

Hi

I have a DNA sequence and I need to analyze this sequence by identifying the regulatory elements found in introns. Is there any software that can predict important DNA signals founf in the internal introns?

By mutagenesis kit (Quikchange II kit). It has been done before with another group and they succeeded in insertion but I was not. I emailed them but there was no reply that is why I posted this problem in this forum....

Hi Jackbean

This is a piece of PCR product which is called megaprimer because I want to insert this piece into my vector.

Hi everyone: I am interested to make a large insertion (780bp) in my vector. This 780 bp is a PCR product (megaprimer), I have used quikchange II mutagenesis kit but couldn't success?? any idea about how to success??? My thinking about the failing in this insertion is in the megaprimer ??? Any sugge...

Hello

I am using Real time PCR for gene expression analysis (cDNA-PCR), can anyone refer me to a reference article with similar work.

No .. I mean CAP site at the RNA LEVEL it is either transcription or translation initiation site. So any one can help???

Hi Everyone

I got confused with the meaning of CAP site.... is it transcription initiation site OR translation initiation site???

I used T-A cloning vector and for the promotor, I used the gene's itself promotor and it showed a nice expression with the mutant but less expression with the wild type. the mutation was a point mutation (single nucleotide substitusion). Any idea for how to make the wild type to be more expressed th...

Hi I am working on in vitro expression analysis. I have transfected a mammalian cell line with a gene of wild type and mutant one. surprisingly, I found that the transcript level of the wild type gene was less than the mutant (which is against the logic theory where the wild type should be expressed...