Search found 658 matches

by blcr11
Fri Jul 10, 2009 10:51 pm
Forum: Molecular Biology
Topic: product-vs-time experiment of amylase
Replies: 3
Views: 3110

Any chance you made a mistake with the substrate (or possibly the enzyme) concentration and you are saturating the enzyme at all substrate levels?
by blcr11
Fri May 08, 2009 8:20 pm
Forum: General Discussion
Topic: Stats help please
Replies: 1
Views: 1358

Re: Stats help please

You sure you don't really have a "two-column" problem with "cased" flow rates vs. "uncased" flow rates; unless the "flow rate" column is some kind of control? I'm imagining the question as: is there a significant difference between cased and uncased flow rates...
by blcr11
Fri Apr 24, 2009 11:52 pm
Forum: Molecular Biology
Topic: Calculating the number of plasmid molecules
Replies: 2
Views: 12399

Re: Calculating the number of plasmid molecules

You have one of the numbers you need, but you kinda got the right answer for the wrong reason, and you haven't determined the mass, you've determined the molecular weight in gm/mole of the 3000 bp plasmid. You've been gven the molecular weight of an average nucleotide (320 gm/mole), but the plasmid ...
by blcr11
Wed Apr 08, 2009 7:20 pm
Forum: Bioinformatics
Topic: Pymol
Replies: 1
Views: 2114

Re: Pymol

There are none that I'm aware of. After the manuals there is a pyMol Wiki with some useful tips and scripts. Then there is a quick tutorial someone at UAB came up with at one point. That's about it.

http://pymolwiki.org/index.php/Main_Page

http://137.189.50.96/kbwong/teaching/py ... orial.html
by blcr11
Thu Apr 02, 2009 7:32 pm
Forum: Molecular Biology
Topic: enzyme question
Replies: 3
Views: 3157

Re: enzyme question

2200 M-1 cm-1 at pH 7
by blcr11
Wed Apr 01, 2009 3:05 pm
Forum: General Discussion
Topic: Borax Crystals
Replies: 5
Views: 28591

Re: Borax Crystals

I think what you are asking, then, is how do crystals form. The physics of crystal formation is essentially the same, whether you're talking about borax crystals in a classroom, or mineral crystallization in molten rock, or stalagmite/stalagtite formation in caves, or protein crystallization in the ...
by blcr11
Mon Mar 23, 2009 10:37 am
Forum: Molecular Biology
Topic: SDS-PAGE
Replies: 2
Views: 8196

Re: SDS-PAGE

Each standard protein will be an (x,y) point on the graph where x = relative mobility and y = log(MW). Once you plot all the standard proteins, you should be able to draw a reasonable line through the points (or do a regression). This is your calibration line. Now find the relative mobility of the u...
by blcr11
Mon Mar 23, 2009 3:13 am
Forum: Human Biology
Topic: Oral Glucose Tolerance Test
Replies: 3
Views: 3357

Re: Oral Glucose Tolerance Test

Plasma is the aqueous phase of blood after the cells have been spun out. There shouldn't be any cells left, if the samples have been processed correctly. In diabetics, especially following ingestion of such a large amount of glucose administered by the OGTT, plasma glucose levels are expected to be ...
by blcr11
Thu Mar 05, 2009 2:42 pm
Forum: Molecular Biology
Topic: Beer Lambert Law
Replies: 1
Views: 9099

Re: Beer Lambert Law

So what happened to your exponent on the power of ten? I didn't check the extinction coefficient (now preferably called the molar absorptivity) of NADH. I'll take your word for it that it is 6.22E3 A/ M cm. What you haven't told anyone is what the volume of your sample of serum was and and if it was...
by blcr11
Mon Feb 23, 2009 4:56 pm
Forum: General Discussion
Topic: Buffer capacity
Replies: 1
Views: 7496

Re: Buffer capacity

Buffer capacity is defined as the number of moles of strong acid or strong base needed to change the pH of the solution by 1 pH unit. The simple experiment would be to measure the pH of a known volume of solution; then add a known amount of strong acid or strong base (HCl or NaOH, typically) and re-...
by blcr11
Wed Feb 18, 2009 5:51 pm
Forum: Genetics
Topic: Hardy-Weinberg / Allele Frequency HELP?
Replies: 2
Views: 7024

I may be saying the same thing as pharmquocdat41191 is, but I think you are confusing q with q^2. When you determine the number of recessive genotypes, you are finding q^2 = 1/3. Try it this way and I think you'll get the right answer.
by blcr11
Wed Jan 14, 2009 7:14 am
Forum: Bioinformatics
Topic: Cell culture-biotechnology--->HELP!
Replies: 2
Views: 4476

Just google "trypsinization" and you will get most of the information you want to know. EDTA is a standard chelator of (forms a soluble complex with) calcium and magnesium ions, both of which inhibit the enzyme trypsin. Cells themselves contain these metal ions and calcium and magnesium sa...