Search found 8 matches

by RastaTings
Mon Apr 09, 2007 3:24 am
Forum: Bioinformatics
Topic: Zymography Question
Replies: 1
Views: 5002

Zymography Question

Ok, so I think this would apply to cell biology (it's from a cell biology class). I recieved this questions on an assignment Ductal elongation in the mammary gland is dependant upon the action of MMPs. In order to identify if MMP3 is active during secondary branching, you prepared extracts from mous...
by RastaTings
Mon Apr 09, 2007 1:23 am
Forum: Genetics
Topic: Distinguishing Gametes & Genotypes
Replies: 4
Views: 2583

Gametes are haploid meiotic products that will impact what genotype the zygote (fertilized egg) will have. Gametes therfore contribute half of the genotype, the total genotype will me made up of the genetic material contributed by 2 gametes. So a genotype will have 2 copies of a gene, while a gamete...
by RastaTings
Sun Apr 08, 2007 8:59 pm
Forum: Genetics
Topic: Quadhybrid/Four Traits Genotype Ratio
Replies: 2
Views: 5154

if there is no linkage, just look at each gene individually and multiply the ratios through
by RastaTings
Sun Apr 08, 2007 8:39 pm
Forum: Cell Biology
Topic: Zymography Question
Replies: 1
Views: 2483

Zymography Question

Ok, so I think this would apply to cell biology (it's from a cell biology class). I recieved this questions on an assignment Ductal elongation in the mammary gland is dependant upon the action of MMPs. In order to identify if MMP3 is active during secondary branching, you prepared extracts from mous...
by RastaTings
Mon Mar 19, 2007 4:57 am
Forum: Cell Biology
Topic: Cell Cycle Problem
Replies: 5
Views: 7392

no you're right I see my mistake. Thank you so much for all your help, you don't know how much I appreciate it
by RastaTings
Sun Mar 18, 2007 11:02 pm
Forum: Cell Biology
Topic: Cell Cycle Problem
Replies: 5
Views: 7392

Ok I think my above idea was wrong. So I have this second one. 1. It's an asynchronous culture, so the proportion of cells initially labelled should correspond to the approximate length of S-phase, so in this case 43/150 x 28 = 8 hours for S-phase 2. The time it takes for the first mitotic cells to ...
by RastaTings
Sun Mar 18, 2007 10:45 pm
Forum: Cell Biology
Topic: Cell Cycle Problem
Replies: 5
Views: 7392

Thanks for replying, I think that helps me get started. What I have so far is because the brdU is going to be used during DNA synthesis it will be S-phase cells that should uptake the brdU (because that's when DNA replication occurs). Based on that here's what I'm thinking; 1. 1.5 hours elapses from...
by RastaTings
Sun Mar 18, 2007 7:59 pm
Forum: Cell Biology
Topic: Cell Cycle Problem
Replies: 5
Views: 7392

Cell Cycle Problem

This is a problem that I have been given for a cellular biology class. I think I can figure it out, if I knew at what stage of the cell cycle the BrdU will first show up. I realize it's a DNA precursor so I'm thinking it will be visible once the Chromosomes condense, or Prophase. Any help at this po...