Search found 55 matches

by Vagabond
Wed Apr 11, 2007 12:41 pm
Forum: General Discussion
Topic: biology master degree
Replies: 14
Views: 9730

I think he is from Israel it says under is avatar. Letters of reference are mainly used to try and get an idea of what other people think of you. If references are from people who happen to be in the field of interest then all the better, but not required. So yes it would be good to get some from pr...
by Vagabond
Tue Apr 10, 2007 12:14 pm
Forum: General Discussion
Topic: GraphPad Prism
Replies: 8
Views: 13761

I believe Excel will graph it for you. As for calculating it off the graph you need an equation. I will look around I know I used to have one in a file somewhere but Now I use Prism, StatView or SoftMax
by Vagabond
Wed Apr 04, 2007 2:53 pm
Forum: Cell Biology
Topic: Current Research Reports
Replies: 4
Views: 3195

If you can not find any PDF on line google should turn up a few (type in a subject). Try a Library they usually carry some scientific journals. If you are close to a university they defiantly will have some.
by Vagabond
Wed Apr 04, 2007 1:38 pm
Forum: General Discussion
Topic: EEG
Replies: 6
Views: 3364

You will be fine but you should have asked before volunteering :)
by Vagabond
Thu Mar 29, 2007 6:26 pm
Forum: General Discussion
Topic: ELISA hormon assay
Replies: 4
Views: 4813

Quickly you plot the OD reading for you standard (say on the Y axis) similar to RIA and then across the X axis you plot you concentrations. Draw the line (curve) get a ruler or calculate the slope of the line and use it to calculate what the OD of you sample is. Follow that down to the X axis and th...
by Vagabond
Tue Mar 20, 2007 12:48 pm
Forum: Cell Biology
Topic: Does trypsin-based cell harvesting damage membrane proteins?
Replies: 1
Views: 2388

Yes Trypsin will damage a lot of membrane bound proteins. I don not know if EGFR itself has a Trypsin recog site though. You could try to physically scrape the cells off.
by Vagabond
Mon Mar 19, 2007 2:55 pm
Forum: Molecular Biology
Topic: Plasmids for beginners...?
Replies: 1
Views: 1349

Check Promega site they have a large list of plasmids and plasmid info. Invitrogen may also be a good starting point.
7,000 is not too large you should not have any major issue in cloning this
pBluescript is a widely used plasmid

GL
by Vagabond
Mon Mar 19, 2007 2:42 pm
Forum: Cell Biology
Topic: Histology Help
Replies: 6
Views: 8557

That is tough,
off the cuff, it looks like it could be a really poorly stained cornea or perhaps the outer layer of skin (Stratum Corneum). But I am not sure of that I will think a little bit more on it.
by Vagabond
Thu Mar 15, 2007 4:27 pm
Forum: Molecular Biology
Topic: Elimination of low-range co-purified proteins
Replies: 3
Views: 2366

Your sizes may be to close for the typical membrane centrifugal filter to separate. They are not as good as they say. Call the tech support to check.

Run a size exclusion column (HPLC - preferred) or by gravity. If gravity you will want a tall column.
by Vagabond
Fri Mar 09, 2007 2:27 pm
Forum: General Discussion
Topic: Modest question
Replies: 5
Views: 2401

I also have absolutely no clue as to what you are asking.
by Vagabond
Thu Mar 08, 2007 3:08 pm
Forum: Cell Biology
Topic: Cell motility/invasiveness assay?
Replies: 2
Views: 2587

They are time consuming I assume you are aware of Matrigel and micro-boyden chambers.

Matrigel would work great for tissue cultures
by Vagabond
Wed Mar 07, 2007 3:42 pm
Forum: Genetics
Topic: Genetics Question
Replies: 7
Views: 4508

Lanes 1, 2 and 3 are different (different sizes) because they are reflecting the different lengths of the dinucleotide repeat (ATATATATAT)n. You state that you have 3 different strains. You state that each strain carries a different length of repeats. Typically the experiment is set up to enzymatica...