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Cellulose Binding Domains (CBD) were conjugated with fluorescein isothiocyanate (FITC).
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- Quantification of the CBD-FITC conjugates surface coating on cellulose fibres
In this work, the surface concentration of CBD adsorbed on cellulose
fibres was estimated. The coating values obtained were higher than
expected, corresponding in theory to several layers (4 to 6) of CBDs
adsorbed on the external surface. However, it has been demonstrated
that the CBDs penetrate the fibres. An important amount of CBDs was
detected inside the fibres by immunolabelling and confocal microscopy.
It seems that a large fraction of the adsorbed CBDs actually penetrate
the fibres. The surface coverage values are, undoubtedly, high enough
to justify a change in the fibres' surface properties. CBDs may
therefore be used as powerful tools to modulate the fibres' surface
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