Tables
- Proteomic characterization of two snake venoms: Naja naja atra and Agkistrodon halys

Table 1   Summary of the identified venomous proteins from Naja (a) and Agki (b) determined by different proteomics approaches

The numbers in parentheses indicate how many proteins were identified by an approach.

(a)
Approach Shotgun-LC-MS/MS (%) 1DE-LC-MS/MS (%) GF-LC-MS/MS (%) GF-2DE-MALDI–TOF-MS (%) All approaches (%)
Venom proteins
Cardiotoxins 56 (44) 70 (46) 78 (60) 56 (68)
Neurotoxins 5 (4) 4 (2) 13 (10) 8 (11)
Haemotoxins 3 (2) 2 (1) 13 (2) 2 (3)
Phospholipases A2 18 (14) 7 (5) 4 (3) 11 (15)
Metalloproteinases
Other proteins 18 (14) 17 (11) 5 (4) 87 (14) 22 (27)
(b)
Approach Shotgun-LC-MS/MS (%) 1DE-LC-MS/MS (%) GF-LC-MS/MS (%) GF-2DE-MALDI–TOF-MS (%) All approaches (%)
Venom proteins
Cardiotoxins 7 (2) 7 (3) 8 (4) 8 (6)
Neurotoxins 2 (1) 1 (1)
Haemotoxins 28 (8) 23 (10) 32 (15) 27 (4) 35 (26)
Phospholipases A2 14 (4) 23 (10) 15 (7) 27 (4) 18 (13)
Metalloproteinases 40 (11) 30 (13) 30 (14) 33 (5) 27 (20)
Other proteins 11 (3) 16 (7) 13 (6) 13 (2) 11 (8)
Table 2   Comparison of the identified venomous proteins and their sequence sources in database of snake proteins

All sequence data from snake venoms, cobra and viper, are from NCBI and EBI. The information of species sources are provided by submitters.

Total identified proteins Sequence source from cobra Sequence source from viper Sequence source from other species
Naja Shotgun-LC-MS/MS 78 70 (90%) 2 (2%) 6 (8%)
1DE-LC-MS/MS 65 57 (87%) 1 (1.5%) 7 (10.7%)
GF-LC-MS/MS 77 76 (98.7%) 1 (1.3%)
GF-2DE-MALDI–TOF-MS 16 5 (31%) 1 (6.3%) 10 (62.5%)
Agki Shotgun-LC-MS/MS 28 1 (3.5%) 26 (93%) 1 (3.5%)
1DE-LC-MS/MS 43 1 (2%) 42 (98%)
GF-LC-MS/MS 47 1 (1.5%) 46 (97%) 1 (1.5%)
GF-2DE-MALDI–TOF-MS 15 14 (93%) 1 (6%)

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