Exhaled breath condensate as a method of sampling airway nitric oxide and other markers of infl ammation
Jia LiuBCDEF, Paul S. ThomasEFG
Faculty of Medicine, University of New South Wales and Department of Respiratory Medicine, Prince of Wales Hospital, Randwick, NSW, Australia
Author’s address: Associate Professor Paul S. Thomas, Department of Respiratory Medicine, Prince of Wales Hospital, Randwick, NSW 2031, Australia, e-mail: [email protected]
Source of support: Departmental sources
Most of the methods of investigating lung diseases have been invasive until the discovery that exhaled nitric oxide can be used as a surrogate marker of airway infl ammation, particularly in asthma. Exhaled nitric oxide (NO) is now established as a marker of airway infl ammation. It has been shown to correlate well with eosinophilic asthmatic airway infl ammation, and to be able to predict decline in asthma control and airway function. Altered levels of NO are also associated with other infl ammatory lung diseases. In addition, polymorphisms of the genes encoding the three nitric oxide synthases are associated with phenotypic differences associated with lung diseases. Exhaled NO is, however, non-specifi c. It is therefore of importance that collecting exhaled breath condensate (EBC) has emerged as a potential tool in the study of pulmonary diseases. The exhaled breath is collected in a cooling system which allows water vapour to condense. The EBC contains a number of mediators relating to the NO pathway, including nitrite as a metabolite of nitric oxide, nitrotyrosine, nitrosothiols plus small molecular mediators associated with oxidative stress, including hydrogen ions, and hydrogen peroxide. In addition, reports are emerging of the detection of larger molecules which not only include leukotrienes, prostaglandins, albumin and other proteins, such as cytokines, but also macromolecules, for example, DNA. EBC is becoming a technique which will allow repeated non-invasive sampling from the respiratory tract thus assisting pulmonary research and possibly the monitoring of lung diseases.
Key words: exhaled breath condensate • infl ammation • nitric oxide • airway disease
Source: Med Sci Monit, 2005; 11(8): MT53-62