- Design of a trans protease lentiviral packaging system that produces high titer virus

List of abbreviations

HIV-1, Human Immunodeficiency Virus Type 1; Pr55Gag, Gag precursor polyprotein, MAp17, matrix protein; CAp24, capsid protein, NCp7, nucleocapsid protein; PR, protease; RT, reverse transcriptase, IN, integrase; RCR, replication competent retrovirus; RCL, replication competent lentivirus; SIN, self-inactivating; GFP, green fluorescent protein; TU, transducing units

Competing interests

Among co-authors, K.A.W. and P.L. are minority stockholders with consulting fee from Genetix Pharmaceuticals.

Authors' contributions

K.A.W. was responsible for the design, cloning, testing, and writing of the manuscript. Z.A. and E.A.C. were responsible for the immunoprecipitation experiments, protein analysis, and contributed to writing the manuscript. P.L. was responsible for the design and writing of the manuscript.


We thank Patricia Morales for excellent technical assistance, Paul Allen for critical reading of the manuscript and Maria Denaro for helpful discussions. This work was supported by a grant from the NIH (RO1 DK065939) and Genetix Pharmaceuticals.

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