- Amyloglucosidase enzymatic reactivity inside lipid vesicles
Liposomes have long been used as carrier systems for the delivery of vaccines, therapeutic drugs and hormones because of easy preparation, good biocompatibility and biodegradability, low toxicity, and commercial availability [1-3]. Efficient functioning of enzymes inside liposomes opens up new possibilities of applications in biocatalysis and bioanalytical tools [4-6]. For example, enzyme-containing vesicles can serve as nanoreactors for biospecific reactions. In such reaction systems specific substrates, which permeate across the vesicle membrane lipid bilayer(s), are converted to products by the entrapped enzymatic catalyst [6,7].
The present work explores the development of novel reactive and stable biocatalytic interfaces for direct conversion of substrates. The reactivity of entrapped enzyme inside either multilamellar vesicles (MLV) or large unilamellar vesicles (LUV) liposomes was examined with respect to externally added substrate. The ultimate goal is to design stable catalytic interfaces that will mediate both chemical transformations and interphase transport for extended periods. The enzyme of choice for these experiments is Amyloglucosidase (E. C. 3. 2. 1. 3). As an industrial catalyst, amyloglucosidase (AMG) is one of the most economically important enzymes widely used in many industries such as baking, detergents, sewage treatment, and natural sweeteners . This enzyme catalyzes both exo-(1–4) and branch-point (1–6)-linkages to produce glucose, providing the primary step in the conversion of agricultural feedstocks to ethanol . However, after completion of each batch of reaction, the product is recovered using processes that denature the enzyme catalyst leading to loss of activity, thus increasing processing costs. To eliminate the disadvantages associated with the use of soluble AMG present in the conventional process, AMG has been immobilized on various carriers in an effort not only to retain catalytic activity for conventional processing, but also to maintain stability for repeated and continuous application [10-13]. Here, the utility of liposomal systems for enzyme stabilization and recycle is experimentally demonstrated, and mathematically described.
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