such as "Introduction", "Conclusion"..etc
The results show that quantitative real-time RT-PCR is a favorable approach to analyze cell-type specific gene expression in Volvox carteri. Our approach not only provides a basis for a detailed analysis of individual, previously unknown Volvox genes of the investigated set of genes but also allows for future analysis of the same set of genes (by using the same primers and other RT-PCR conditions) with respect to inducibility by stress, wounding, deficiency or abundance of nutritional compounds, or response on the presence of the sex-inducer (the trigger of sexual development in V. carteri). Furthermore, it allows a characterization of the transcription of these genes in the life cycle (probably without separating cell-types because a separation of cell types from embryos or juveniles can not be achieved earlier than 18–20 h after the onset of embryogenesis, and embryonic and parent somatic cells can't be separated from each other even later . It is also possible to repeat these experiments using developmental or metabolic mutants instead. Finally, this approach can also be extended to a much larger number of genes. We hope that our analysis of cell-type specific expression of almost 40 genes was able to stimulate discussion about the application of a genome-wide expression analysis in Volvox in order to reveal the complete germ-soma program of this fascinating green alga.
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